Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury

XU Feng; et al

doi:10.13406/j.cnki.cyxb.2004.02.004

您当前的位置：

首页 >

文章列表页 >

Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury

更新时间：2024-01-17

- Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury
- Journal of Chongqing Medical University Issue 2, Pages: 131-133(2004)
- 作者机构：
  
  1. 重庆医科大学儿科学院PICU
  2. 重庆医科大学儿科学院PICU,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.02.004
  CLC： R563.8
- Published：2004，
扫描看全文
[1]许峰,何勇,匡凤梧,卢仲毅,王兴勇.急性肺损伤大鼠肺表面活性物质及肺表面活性物质蛋白的研究[J].重庆医科大学学报,2004(02):131-133+152.

XU Feng, et al. Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury. [J]. Journal of Chongqing Medical University (2):131-133(2004)
[1]许峰,何勇,匡凤梧,卢仲毅,王兴勇.急性肺损伤大鼠肺表面活性物质及肺表面活性物质蛋白的研究[J].重庆医科大学学报,2004(02):131-133+152. DOI： 10.13406/j.cnki.cyxb.2004.02.004.

XU Feng, et al. Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury. [J]. Journal of Chongqing Medical University (2):131-133(2004) DOI： 10.13406/j.cnki.cyxb.2004.02.004.

摘要

目的 :探讨肺表面活性物质（PS）及肺表面活性物质蛋白（SP）在内毒素（LPS）性急性肺损伤（ALI）中的变化趋势。方法 :成年Wistar大鼠随机分为对照组（NS组）和肺损伤组（ALI组）

ALI组采用LPS诱导建立ALI模型。用定磷法和高效液相色谱法动态监测了NS组和ALI组第 1、3、5、7h大鼠支气管肺泡灌洗液（BALF）中的总磷脂（TPL）和磷脂各组分

采用逆转录聚合酶链反应（RT -PCR）的方法检测肺组织SP -A、SP -BmRNA的表达情况

此外对肺损伤的病理学改变也进行了对比分析。结果 :与NS组比较

ALI组有明显水肿、出血和炎症。BALF中TPL在 1、3、5、7h明显低于NS组（P <0 .0 5 ）

卵磷酯胆碱（PC）在 3、5、7h明显低于NS组（P <0 .0 5 ） ;而溶血卵磷脂（LPC）在 1、3、5、7h明显高NS组（P <0 .0 5 ）。肺组织SP -A、SP -BmRNA表达在 3、5、7h明显低于NS组（P <0 .0 5 ）。结论 :ALI存在PS的代谢改变

以及SP -A、SP -BmRNA的表达降低

PS含量的降低和组分的改变是导致难以纠正的低氧血症重要原因之一。

Abstract

Objective:To study the alteration trend of pulmonary surfactant(PS) and surfactant-associated protein(SP) in rats with LPS-induced acute lung injury(ALI).Methods:Fifty-six adult Wistar rats were randomly divided into normal saline(NS) group and ALI group.The levels of mRNA of the SP-A

SP-B were measured by reverse transcription polymerase chain reaction(RT-PCR) during iv LPS at 1

7h.The content and component of PS in the bronchoalveolar lavage fluid (BALF) were measured by high performance liquid chromatography (HPLC).In addition

studies on lung dry/wet weight ratio

the protein content of BALF

alveolar oxygen partial pressure (PaO 2)and histological changes were performed.Results:Compared with the NS group

ALI group developed severe lung damage.Edema

hemorrharge and inflammatory changes were found.Total phospholipids (TPL) of BALF at 1

7h were lower than those in the NS group( P <0.05);phosphatidylcholine(PC) at 3

7h were lower than those in the NS group( P <0.05);while lysophosphatidylcholine (LPC) at 1

7h were higher than thosein the NS group(P <0 .0 5 ) .Theexpression ofSP -A

SP -Bm RNAat 3

7h were less than thosein the NSgroup(P <0 .0 5 ) .Conclusion :The changed metabolism of PSex ists in the pathogenesisn of ALI

which is mainly de monstratedas that the decreasein TPL

will de crease expression of SP -A

SP -Bm RNA .Decreasein content and changein components of PS may play an important role in severe hypoxemia in ALI .

关键词

急性肺损伤肺表面活性物质大鼠

Keywords

acute lung injuryPulmonary surfactantrat

references

Views

285

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

TNF-α downregulates the expression of tight junction proteins ZO-1 and Claudin-5 in rat pulmonary microvascular endothelial cells

Research progress of autophagy in lung inflammatory diseases

Histopathological changes and pathological basis of the cryptorchidtestis in rats by maternal exposure to flutamide

DISPOSITION OF ~3H-PIPERAZINE FERULATE IN RATS

PHA INDUCED RAT T-LYMPHOCYTE TRANSFOR MATION IN VIVO

Related Author

Bai Jijia

Shi Guocui

Ma Shenmao

Ma Xigang

Duan Yushan

Liu Qiong

Peng Jinpu

Zhang Deying

Related Institution

Department of Critical Care Medicine,General Hospital of Ningxia Medical University

Department of Respiratory Medicine,Cangzhou People's Hospital

Department of Emergency and Critical Care Medicine,The First Affiliated Hospital of Chongqing Medical University

Department of Urology,the Children’s Hospital,Chongqing Medical University,Ministry of Education Key Laboratory of Child Development and Disorders,Key Laboratory of Pediatrics in Chongqing,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders

重庆医科大学第一医院临床药理研究室

⁰