Experimental study on the relationship between protein kinase C isoforms β、δ and diabetic retinopathy

ZHOU Bo; et al

doi:10.13406/j.cnki.cyxb.2004.02.001

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Experimental study on the relationship between protein kinase C isoforms β、δ and diabetic retinopathy

更新时间：2024-01-17

- Experimental study on the relationship between protein kinase C isoforms β、δ and diabetic retinopathy
- Journal of Chongqing Medical University Issue 2, Pages: 121-123(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院内分泌科
  2. 重庆医科大学基础医学院生化研究室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.02.001
  CLC： R587.2
- Published：2004，
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[1]周波,汪恕萍,曾昭淳.蛋白激酶C同工酶β、δ与糖尿病视网膜病变关系的实验研究[J].重庆医科大学学报,2004(02):121-123+137.

ZHOU Bo, et al. Experimental study on the relationship between protein kinase C isoforms β、δ and diabetic retinopathy. [J]. Journal of Chongqing Medical University (2):121-123(2004)
[1]周波,汪恕萍,曾昭淳.蛋白激酶C同工酶β、δ与糖尿病视网膜病变关系的实验研究[J].重庆医科大学学报,2004(02):121-123+137. DOI： 10.13406/j.cnki.cyxb.2004.02.001.

ZHOU Bo, et al. Experimental study on the relationship between protein kinase C isoforms β、δ and diabetic retinopathy. [J]. Journal of Chongqing Medical University (2):121-123(2004) DOI： 10.13406/j.cnki.cyxb.2004.02.001.

摘要

目的 :探讨糖尿病视网膜病变（DR）发病过程中视网膜血管蛋白激酶C（PKC）同工酶 β、δ表达变化及其与DR发生、发展的关系。方法 :将实验动物随机分为正常对照组及链脲菌素诱导的糖尿病组。采用渗透休克法获取大鼠视网膜血管。在成模后 2周、2月、4月、6月

采用Westernblot法检测视网膜血管PKC同工酶 β、δ表达情况。毛细血管床形态立体定量评估DR。结果 :PKC - β在糖尿病大鼠视网膜血管 2周、2月、4月、6月时呈胞膜至胞浆部移位激活

而PKC -δ在糖尿病大鼠视网膜血管 2周、2月、4月、6月时呈胞膜至胞浆部移位激活。成模 6月后

视网膜深层毛细血管呈现周细胞减少、基底膜增厚的特征性改变

而成模 4月时

电镜显示糖尿病大鼠深层毛细血管基底膜增厚。结论 :在DR发病过程中的不同阶段

PKC同工酶 β、δ在视网膜血管的变化不同

对DR的发生、发展有着不同的调控效应。

Abstract

Objective:To investigate the expression of protein kinase C (PKC) isoforms β

δ in diabetic rats and evaluate the relation between altered PKC β

δ expression and the pathogenesis of diabetic retinopathy (DR).Methods:Wistar rats were randomly divided into control group and diabetic group induced by streptozotocin;osmotic shock method was used to isolate the retinal vessels of rats;and PKC isoforms β

δ espression were determined after 2 weeks

2 months

4 months and 6 months follw-up.DR was assessed by quantitative morphometry of capillary bed.Results:Diabetes could induce the translocation of PKC isoform β from cytosol to membrane pool after 2 weeks

2 months

4 months and 6 months

but δ isoform translocated from membrane to cytosol pool in retinal vessels of diabetic rats after 2 weeks

2 months

4 months and 6 months.Diabetic rats showed pericyte decrease and capillary basement thickening in deep capillary bed of retina after 6 months follow-up.Conclusion:The expression of PKC isoforms β

δ varies in different stages of DR

and the effect of PKC isoforms on the pathogenesis of DR is different.

关键词

蛋白激酶C同工酶视网膜糖尿病

Keywords

Protein kinase CIsoformsVesseldiabetes mellitus

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