Development of ischemic preconditioning model of cultured neonatal rat cardiomyocyte in vitro

HAN Junyong; et al

doi:10.13406/j.cnki.cyxb.2004.01.014

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Development of ischemic preconditioning model of cultured neonatal rat cardiomyocyte in vitro

更新时间：2024-01-17

- Development of ischemic preconditioning model of cultured neonatal rat cardiomyocyte in vitro
- Journal of Chongqing Medical University Issue 1, Pages: 45-47(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院心内科
  2. 重庆医科大学临床学院心内科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.01.014
  CLC： R541
- Published：2004，
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[1]韩君勇,陈运贞.大鼠离体心肌细胞晚期“缺血”预处理模型的建立[J].重庆医科大学学报,2004(01):45-47.

HAN Junyong, et al. Development of ischemic preconditioning model of cultured neonatal rat cardiomyocyte in vitro. [J]. Journal of Chongqing Medical University (1):45-47(2004)
[1]韩君勇,陈运贞.大鼠离体心肌细胞晚期“缺血”预处理模型的建立[J].重庆医科大学学报,2004(01):45-47. DOI： 10.13406/j.cnki.cyxb.2004.01.014.

HAN Junyong, et al. Development of ischemic preconditioning model of cultured neonatal rat cardiomyocyte in vitro. [J]. Journal of Chongqing Medical University (1):45-47(2004) DOI： 10.13406/j.cnki.cyxb.2004.01.014.

摘要

目的 :探讨建立一种方便、实用的离体大鼠心肌细胞“缺血”预处理模型。方法 :实验分为 4组（对照组 1、对照组 2、“缺血”预处理组 1、“缺血”预处理组 2 ）

将原代培养的新生大鼠心肌细胞置入一个改造的密闭保鲜盒内

通入 95 %N2 和 5 %CO2 混合气（预处理 1）或 10 0 %N2 （预处理 2 ）

进行“缺血”预处理

以LDH释放和台盼蓝排斥试验判断心肌细胞损伤程度。结果 :“缺血”预处理组 1的LDH释放较对照组 1少、细胞存活率高（P <0 .0 1） ;“缺血”预处理组 2的LDH释放较对照组 2少、细胞存活率高（P <0 .0 1） ;“缺血”预处理组 1与“缺血”预处理组 2的LDH释放、细胞存活率无差异（P >0 .0 5 ）。结论 :本文建立了一种方便、实用的大鼠离体心肌细胞“缺血”预处理模型

为进一步研究预处理的心肌保护作用提供有效的方法。

Abstract

Objective:To make a convenient and practical ischemic preconditioning model in cultured neonatal rat ventricular myocytes.Methods:The cultured cardiomyocytes dissociated from neonatal rat ventricle were placed in a modified chamber and mixture gas(95%N 2

5%CO 2)or 100% N 2 was put inside.The injury of cardiomyocytes was assessed by LDH release and trypan blue exclusion in four groups(n=6 in each)—Group control-1

Group IPC-1

Group control-2 and Group IPC-2.Results:The release of LDH was lower and cell viability was higher in Group IPC-1 than Group control-1.Similar results were obtained in Group IPC-2 compared with Group control-2.The difference in the release of LDH and cell viability was not significant between Group IPC-1 and Group IPC-2 (P>0.05).Conclusion:The isehemic preconditioning model in vitro may be induced easily by oxygen deprivation in cultured neonatal cardiomyocytes

and can be used to further studyon the mechanism of the late phase of preconditioning.

关键词

心肌细胞缺血预处理培养模型

Keywords

Ischemic preconditioningCardiomyocyteCulturemodel

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