Experimental study on IL-3 gene expression induced by TSPG

WANG Lu; et al

doi:10.13406/j.cnki.cyxb.2004.01.010

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Experimental study on IL-3 gene expression induced by TSPG

更新时间：2024-01-17

- Experimental study on IL-3 gene expression induced by TSPG
- Journal of Chongqing Medical University Issue 1, Pages: 31-33(2004)
- 作者机构：
  
  1. 重庆医科大学基础医学院组胚教研室
  2. 重庆医科大学基础医学院组胚教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.01.010
  CLC： R285
- Published：2004，
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[1]王璐,王亚平.人参总皂甙诱导IL-3基因表达的实验研究[J].重庆医科大学学报,2004(01):31-33+40.

WANG Lu, et al. Experimental study on IL-3 gene expression induced by TSPG. [J]. Journal of Chongqing Medical University (1):31-33(2004)
[1]王璐,王亚平.人参总皂甙诱导IL-3基因表达的实验研究[J].重庆医科大学学报,2004(01):31-33+40. DOI： 10.13406/j.cnki.cyxb.2004.01.010.

WANG Lu, et al. Experimental study on IL-3 gene expression induced by TSPG. [J]. Journal of Chongqing Medical University (1):31-33(2004) DOI： 10.13406/j.cnki.cyxb.2004.01.010.

摘要

目的 :研究人参总皂甙（TSPG）对人早期造血生长因子IL - 3基因表达的影响。方法 :采用逆转录聚合酶链反应（RT -PCR）、核酸分子原位杂交方法。结果 :未经诱导的人T淋巴瘤细胞株—Jurkat细胞RNA提取物的RT -PCR扩增产物中未见IL - 3条带

而经TSPG诱导后Jurkat细胞RNA提取物的RT -PCR扩增产物中出现IL - 3条带。核酸分子原位杂交显示经TSPG诱导的胎儿胸腺细胞、脾细胞IL - 3mRNA表达的阳性率及阳性强度较对照组均有显著提高。结论 :人参总皂甙能诱导人淋巴细胞表达IL - 3mRNA

从而促进人早期血细胞发生。

Abstract

Objective:To explore the effect of TSPG (total saponins of panax ginseng) on gene expression of IL-3 which is one of the important hematopoietic growth factor at early stage of hematopoiesis.Methods:Techniques of RT-PCR and in situ hybridization were used.Results:A band corresponding to IL-3 was observed after RNA extraction from TSPG stimulated Jurkat cells were subjected to RT-PCR

while such band was not observed after RNA extraction from unstimulated Jurkat cells were subjected to RT-PCR.Comparing with the control group

both quantity and intensity of IL-3mRNA expression in fetus thymocytes and splenocytes induced by TSPG were obviously promoted.Conclusion:TSPG can induce human lymphocytes to express IL-3 mRNA

thus promote human hematopoiesis at early stage.

关键词

人参总皂甙造血生长因子白细胞介素-3基因表达

Keywords

Total saponins of panax ginsengHematopoietic growth factorInterleukin-3gene expression

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