Application of single nucleotide polymorphism in the study of the susceptible gene of microangiopathy in diabetes mellitus

ZHANG Yuhong; et al

doi:10.13406/j.cnki.cyxb.2004.01.006

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Application of single nucleotide polymorphism in the study of the susceptible gene of microangiopathy in diabetes mellitus

更新时间：2024-01-17

- Application of single nucleotide polymorphism in the study of the susceptible gene of microangiopathy in diabetes mellitus
- Journal of Chongqing Medical University Issue 1, Pages: 18-20(2004)
- 作者机构：
  
  1. 重庆医科大学临床学院检验科
  2. 重庆医科大学临床学院内分泌科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.01.006
  CLC： R587.2
- Published：2004，
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[1]张玉洪,肖谦.单核苷酸多态性在研究糖尿病微血管病变易感基因中的应用[J].重庆医科大学学报,2004(01):18-20.

ZHANG Yuhong, et al. Application of single nucleotide polymorphism in the study of the susceptible gene of microangiopathy in diabetes mellitus. [J]. Journal of Chongqing Medical University (1):18-20(2004)
[1]张玉洪,肖谦.单核苷酸多态性在研究糖尿病微血管病变易感基因中的应用[J].重庆医科大学学报,2004(01):18-20. DOI： 10.13406/j.cnki.cyxb.2004.01.006.

ZHANG Yuhong, et al. Application of single nucleotide polymorphism in the study of the susceptible gene of microangiopathy in diabetes mellitus. [J]. Journal of Chongqing Medical University (1):18-20(2004) DOI： 10.13406/j.cnki.cyxb.2004.01.006.

摘要

目的 :检测人醛糖还原酶基因启动区（C - 10 6T）单核苷酸多态性及其基因型在重庆汉族人 2型糖尿病中的分布

并分析与糖尿病微血管病变易感性的关系。方法 :用聚合酶链反应扩增上游启动区

再用限制性内切酶BfaI进行多态性分析 2型糖尿病人的（C - 10 6T）基因型分布频率。结果 :2型糖尿病微血管病变患者较无微血管病变并发症的糖尿病人的CC基因型频率显著增加TT基因型频率显著减少（χ2 分别为 6 .78和 4 .4 5

P <0 .0 1和P <0 .0 5 ）。结论 :AR基因启动区（C - 10 6T）单核苷酸多态性与中国汉族 2型糖尿病患者微血管病变的发生有一定的关系

可能是糖尿病微血管病变的功能性多态性和遗传标志之一。

Abstract

Objective:To detect the distribution of the (C-106T) single nucleotide polymorphism and genotype of promoter region of aldose reductase gene and to study its relation to the susceptibility of microangiopathy in type 2 diabetes mellitus in Chinese Han from Chongqing.Methods:The aldose reductase gene in promoter region was amplified by polymerase chain reaction and the frequency of genotype of type 2 diabetes were analyzed by restriction enzyme BfaI.Results:The frequencies of genotype CC were significantly higher and the frequencies of genotype TT were much lower in type 2 diabetes patients with microangiopathy than in those without microangiopathy (χ 2=6.78 and 4.45

respectively

P<0.01 and P<0.05).Conclusion:This finding suggests that (C-106T) single nucleotide polymorphism in the aldose reductase gene promoter region may be associated with diabetic microangiopathy of Chinese Han type 2 diabetes.It may be a functional polymorphism and can serve as one of inherited marker for susceptibility of diabetic microangiopathy.

关键词

2型糖尿病微血管病变醛糖还原酶基因单核苷酸多态性

Keywords

type 2 diabetes mellitusDiabetic microangiopathyAldose reductase genesingle nucleotide polymorphism

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