Detection of Chlamydia trachomatis DNA by gap ligase chain reaction (G-LCR)

WEI Hong; et al

doi:10.13406/j.cnki.cyxb.2004.01.003

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Detection of Chlamydia trachomatis DNA by gap ligase chain reaction (G-LCR)

更新时间：2024-01-17

- Detection of Chlamydia trachomatis DNA by gap ligase chain reaction (G-LCR)
- Journal of Chongqing Medical University Issue 1, Pages: 8-10(2004)
- 作者机构：
  
  1. 重庆医科大学儿科学院新生儿科
  2. 重庆医科大学儿科学院新生儿科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.01.003
  CLC： R346
- Published：2004，
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[1]韦红,吴仕孝,杨军,余加林,伍金林,刘官信.缺口连接酶链反应检测沙眼衣原体DNA的实验研究[J].重庆医科大学学报,2004(01):8-10.

WEI Hong, et al. Detection of Chlamydia trachomatis DNA by gap ligase chain reaction (G-LCR). [J]. Journal of Chongqing Medical University (1):8-10(2004)
[1]韦红,吴仕孝,杨军,余加林,伍金林,刘官信.缺口连接酶链反应检测沙眼衣原体DNA的实验研究[J].重庆医科大学学报,2004(01):8-10. DOI： 10.13406/j.cnki.cyxb.2004.01.003.

WEI Hong, et al. Detection of Chlamydia trachomatis DNA by gap ligase chain reaction (G-LCR). [J]. Journal of Chongqing Medical University (1):8-10(2004) DOI： 10.13406/j.cnki.cyxb.2004.01.003.

摘要

目的 :建立缺口连接酶链反应方法检测沙眼衣原体。方法 :根据编码CT主要外膜蛋白的基因（ompl）的稳定区设计2对互补的寡核苷酸探针。采用G -LCR和常规PCR扩增CT标准株DNA

并对二者检测CT的敏感性进行比较。结果 :对5种CT标准株进行G -LCR扩增

均出现 5 4bp长度的DNA片段。敏感性实验可检测出 2个CT原体

而PCR可检测出2 0EBs。G -LCR较PCR敏感 10倍 ;在特异性方面

不扩增肺炎衣愿体及其他细菌DNA

具有很高的特异性。结论 :G -LCR用于检测沙眼衣原体特异、敏感、快速、准确

可为CT感染的临床诊断提供依据。

Abstract

Objective:To develop a new nucleic amplication method for detection of Chlamydia trachomatis DNA by gap ligase chain reaction(G-LCR).Methods:A G-LCR DNA amplification assay that targeted the outer major membrane protein gene(omp1)of CT was established to detect CT infection.The sensitivity and specificity of a newly developed G-LCR test was examined by the use of highly purified elementary bodies (EBs).DNA fragments of different species and from other bacteria1 were detected with G-LCR and routine polymerase chain reaction (PCR).Results:Using G-LCR

DNA fragments of 54bp were amplified from five different species.The sensitivity could be improved to detect out 2 chlamydial elementary bodies.G-LCR detected ten-fold EBs than PCR.No signal was observed when C.pneumoniae and other bacteria were used as templates.Conclusion:G-LCR is sensitive

rapid and specific for detection of Chlamydia trachomatis.

关键词

沙眼衣原体连接酶链反应聚合酶链反应

Keywords

Chlamydia trachomatisLigase chain reactionPolymerase chain reaction

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Department of Neonatology,the Children’s Hospital,Chongqing Medical University

重庆医科大学基础医学院微生物学教研室重庆医科大学基础医学院微生物学教研室讲师

重庆医科大学基础医学院微生物学教研室讲师

重庆医科大学基础医学院微生物学教研室

Department of Otolaryngology, College of Clinical Medicine

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