Detection of CK20 in peripheral blood of patients with transitional cell carcinoma of the bladder

HAN Yonghua; et al

doi:10.13406/j.cnki.cyxb.2003.06.015

您当前的位置：

首页 >

文章列表页 >

Detection of CK20 in peripheral blood of patients with transitional cell carcinoma of the bladder

更新时间：2024-01-17

- Detection of CK20 in peripheral blood of patients with transitional cell carcinoma of the bladder
- Journal of Chongqing Medical University Issue 6, Pages: 735-737(2003)
- 作者机构：
  
  1. 重庆医科大学临床学院泌尿外科
  2. 重庆医科大学临床学院泌尿外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.06.015
  CLC： R737.14
- Published：2003，
扫描看全文
[1]韩永华,吴小候.膀胱移行细胞癌患者外周血CK20表达的研究[J].重庆医科大学学报,2003(06):735-737.

HAN Yonghua, et al. Detection of CK20 in peripheral blood of patients with transitional cell carcinoma of the bladder. [J]. Journal of Chongqing Medical University (6):735-737(2003)
[1]韩永华,吴小候.膀胱移行细胞癌患者外周血CK20表达的研究[J].重庆医科大学学报,2003(06):735-737. DOI： 10.13406/j.cnki.cyxb.2003.06.015.

HAN Yonghua, et al. Detection of CK20 in peripheral blood of patients with transitional cell carcinoma of the bladder. [J]. Journal of Chongqing Medical University (6):735-737(2003) DOI： 10.13406/j.cnki.cyxb.2003.06.015.

摘要

目的 :探讨CK2 0在膀胱移行细胞癌（TCCB）患者外周血的表达及其意义。方法 :采用逆转录聚合酶链反应（RT -PCR）检测 6 3例TCCB患者和 19例健康志愿者以及 19例非肿瘤患者外周血CK2 0表达。以GAPDH作为内参照。结果 :19例健康志愿者和 19例非肿瘤患者外周血CK2 0的表达均为阴性 ;6 3例TCCB患者外周血CK2 0阳性表达率为 2 5 .4 % （16 /6 3）

其中T1期为 0 （0 /31）

T2 期为 12 .5 % （2 /16 ）

T3 期为 77.8% （7/9）

T4期为 10 0 % （7/7）

CK2 0阳性表达率随分期增加而增加

T3～ 4期明显高于T1期和T2 期。对T1～ 3 期 33例患者随访 6个月

其中外周血CK2 0阴性表达 2 6例未发现远处转移 ;外周血CK2 0阳性表达 7例中 2例发生远处转移。结论 :检测外周血CK2 0表达

可以提示癌细胞的血行播散

对判断预后以及指导治疗具有一定临床价值。

Abstract

Objective:To study the expression of cytokeratin (CK) 20 in the peripheral blood of patients with transitional cell carcinoma of the bladder and to evaluate its clinical significance.Methods:CK20 mRNA in 63 patients with TCCB

19 non-cancerous patients and 19 healthy volunteers was detected respectively.GAPDH was used as a general marker for normal blood cells.CK20 and GAPDH were amplified by using RT-PCR.Results:Nineteen healthy volunteers and 19 non-cancerous patients showed no CK20 signals in peripheral blood.Sixteen of 63(25.4%) patients with TCCB showed positive CK20 signals in peripheral blood

including 0/31 of stage T 1

2/16 of stage T 2

7/9 of stage T 3

7/7 of stage T 4.The CK20 expression difference between T 1/T 2 group and T 3-4 groups was significant.Two patients with positive CK20 signals in peripheral blood had distant metastases during a 6-month follow-up period.Conclusion:CK20 is applicable to detect cancer cells in the peripheral blood of transitional cell carcinoma of the bladder and may play an important role in the evaluation of the prognosis and postoperative management of TCCB.

关键词

膀胱肿瘤CK20逆转录聚合酶链反应血

Keywords

Bladder tumorPeripheral bloodRT-PCRCK20

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The study of bladder cancer cell line irradiated with high intensity focused ultrasound

Clinical and pathological analysis of bladder sarcomatoid carcinomas

Experimental study on the effects of Mad1 to bladder tumor in vivo

Research allicin allied with DDP on growth inhibition and induction apoptosis of rat bladder tumor

The clinical-pathogical significance and the expression of maspin mRNA and protein in human gastric carcinoma

Related Author

王智彪

伍烽

张唯力

杨光永

et al

LIU Chuan

林艳君

HU Zili

Related Institution

重庆医科大学医学超声工程研究所

Department of Urology, the Second College of Clinical Medicine , Chongqing Medical University

重庆医科大学第二临床学院泌尿外科

重庆医科大学第二临床学院病理科

⁰