Objective:To find out the specific gene related to heroin dependence.Methods:Heroin-dependent models in mice were established by dose-increasing administration.After withdrawal syndrome was precipitated by naloxone

total RNA and mRNA were isolated from the brains of experimental and control groups respectively

then suppressive subtractive hybridization was proceeded.The hybridization sample was amplified by PCR and analyzed on a agarose/EB gel.Results:The mRNA level of the experimental group was significantly higher than that of control group and a differently expressing cDNA fragment was obtained from the brain of the experimental group.Conclusion:There is probably an expression of the specific gene in the brain of heroin-dependent mice.