Objective:To investigate the expressions of NFκB and IL-6 in vascular endothelial cells with treatment of different oxidized lipoproteins; the different expressions of PPARα

PPARγ and NFκB when incubated with oxidized lipoproteins.Methods:HUVECs were isolated from fresh umbilical cords obtained at normal deliveries.THP-1 cell line was commercially purchased. The lipoproteins were obtained by density-gradient ultracentrifugation and were oxidized through incubation with 15μmol/L CuSO 4. The different response extent of transcription factors and cytokine factors among four different oxidized lipoproteins were observed through immunohistochcmistry

FACS

and ELISA.The would-be unbalanced expressions of three transcription factors PPARα

PPARγ and NFκB were confirmed by immunohistochemistry and FACS.Results:There were some differences among the four kinds of oxidized lipoproteins to activate the transcription factor and inflammatory factors in the EC and THP.oxLp(a) had the strongest effect.The proinflammation transcription factor NFκB has been far more vigorously activated than PPARs when encountered with oxidized lipoproteins. Their expressions were all enhanced with time.Conclusion:The different patterns of lipoproteins vary in activating the nuclear transcription factors and the inflammation factors. Unbalanced activation between PPARs and NFκB exists with the incubation of oxidized lipoproteins in vascular endothelial cells.