Effect of total saponins of Panax ginseng on K562 cells in expressing clusters of differentiation antigen and hematopoietic growth factor recepters

CHEN Tingmei; et al

doi:10.13406/j.cnki.cyxb.2003.05.004

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Effect of total saponins of Panax ginseng on K562 cells in expressing clusters of differentiation antigen and hematopoietic growth factor recepters

更新时间：2024-01-17

- Effect of total saponins of Panax ginseng on K562 cells in expressing clusters of differentiation antigen and hematopoietic growth factor recepters
- Journal of Chongqing Medical University Issue 5, Pages: 568-570(2003)
- 作者机构：
  
  1. 重庆医科大学检验系临床免疫学教研室
  2. 重庆医科大学基础医学院组胚教研室
  3. 重庆医科大学研究生处,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.05.004
  CLC： R285
- Published：2003，
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[1]陈婷梅,王亚平,陈地龙.人参皂甙对K562细胞表面分化抗原及造血生长因子受体表达的影响[J].重庆医科大学学报,2003(05):568-570.

CHEN Tingmei, et al. Effect of total saponins of Panax ginseng on K562 cells in expressing clusters of differentiation antigen and hematopoietic growth factor recepters. [J]. Journal of Chongqing Medical University (5):568-570(2003)
[1]陈婷梅,王亚平,陈地龙.人参皂甙对K562细胞表面分化抗原及造血生长因子受体表达的影响[J].重庆医科大学学报,2003(05):568-570. DOI： 10.13406/j.cnki.cyxb.2003.05.004.

CHEN Tingmei, et al. Effect of total saponins of Panax ginseng on K562 cells in expressing clusters of differentiation antigen and hematopoietic growth factor recepters. [J]. Journal of Chongqing Medical University (5):568-570(2003) DOI： 10.13406/j.cnki.cyxb.2003.05.004.

摘要

目的 :进一步探讨人参皂甙（TSPG）诱导K5 6 2细胞分化的作用机制及了解K5 6 2细胞的生物学特点

为开发TSPG临床应用提供理论与实验依据。方法 :采用形态学观察和流式细胞术等方法

研究TSPG在诱导K5 6 2细胞向较为成熟细胞分化过程中

对其表面分化抗原及造血生长因子受体表达的影响。结果 :TSPG可诱导K5 6 2细胞向较成熟的红系、粒系细胞分化

作用后细胞表达CD15、HIR2 、EPO -R和GM -CSF -R的阳性细胞率均有所升高。结论 :K5 6 2细胞为分化受阻滞的异常造血祖细胞

其生物学特点与正常人多向造血祖细胞（CFU -Mix）相似 ;TSPG诱导K5 6 2细胞成熟分化的作用机理可能与TSPG促进K5 6 2细胞EPO -R、GM -CSF -R表达升高有关。

Abstract

Objective:To clarify further the mechanisms for total saponins of Panax ginseng(TSPG)in inducing differentiation of K562 cells

and to provide the theoretical basis and the experimental evidence of its clinical application.Methods:By using cell culture in vitro

morphological observation and flow cytometry

the effect of TSPG on K562 cells in expressing CD 15

HIR 2

EPO-R and GM-CSF-R were studied.Results:The results indicated that TSPG could induce the differentiation of K562 cells toward proerythroid cells and progranulocytic cells.It was also shown that after induction by TSPG

the ratio of positive K562 cells expressing CD 15

HIR 2

erythropoietin recepter(EPO-R) and granulocyte-macrophage colony stimulating facter recepter(GM-CSF-R) increased.Conclusion:K562 cells are abnormal cells whose differentiation are blocked

but its biological features are similar to the normal hematopoietic stem cells.The mechanism for TSPG inducing differentiation of K562 cells may be related to the higher expression of EPO-R and GM-CSF-R.

关键词

人参总皂甙K562CD15HIR2EPO受体GM-CSF受体

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Department of Hematology, College of Clinical Medicine,Chongqing Medical Universitry

重庆医科大学基础医学院组织胚胎学教研室

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