Experimental study on embryo culturing medium of malignant tumor growth fluid

YANG Zhu; et al

doi:10.13406/j.cnki.cyxb.2003.05.003

您当前的位置：

首页 >

文章列表页 >

Experimental study on embryo culturing medium of malignant tumor growth fluid

更新时间：2024-01-17

- Experimental study on embryo culturing medium of malignant tumor growth fluid
- Journal of Chongqing Medical University Issue 5, Pages: 564-567(2003)
- 作者机构：
  
  1. 重庆医科大学第二临床学院妇产科
  2. 重庆医科大学超声工程研究所,重庆,400010
  3. 重庆医科大学实验动物中心
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.05.003
  CLC： R73-36
- Published：2003，
扫描看全文
[1]杨竹,赵颉,胡丽娜,谭毅,王智彪.恶性肿瘤细胞生长液培养早期胚胎的实验研究[J].重庆医科大学学报,2003(05):564-567.

YANG Zhu, et al. Experimental study on embryo culturing medium of malignant tumor growth fluid. [J]. Journal of Chongqing Medical University (5):564-567(2003)
[1]杨竹,赵颉,胡丽娜,谭毅,王智彪.恶性肿瘤细胞生长液培养早期胚胎的实验研究[J].重庆医科大学学报,2003(05):564-567. DOI： 10.13406/j.cnki.cyxb.2003.05.003.

YANG Zhu, et al. Experimental study on embryo culturing medium of malignant tumor growth fluid. [J]. Journal of Chongqing Medical University (5):564-567(2003) DOI： 10.13406/j.cnki.cyxb.2003.05.003.

摘要

目的 :寻找一种体外胚胎培养的良好培养液。方法 :小鼠交配后第 4天取胚胎

分别用肾癌细胞株GRC - 1的生长液和基础胚胎培养液（FD +2 0 %胎牛血清）进行体外培养

观察胚胎的脱带率、贴壁率和扩展率。结果 :肾癌细胞株GRC - 1的生长液可使体外培养胚胎的脱带率、贴壁率和扩展率分别达到 89.6 8%、86 .5 1%和 84 .92 %

远远高于对照组的脱带率、贴壁率和扩展率（P <0 .0 1）。结论 :肾癌细胞株GRC - 1的生长液对体外胚胎培养具有明显的促进作用。

Abstract

Objective:To find a good culture medium for embryo development in-vitro.Methods:D4 mouse embryo was cultured with carcinoma of kidney GRC-1 growth fluid and FD+20% fetal calf serum respectively in-vitro and the rate of zona-hatching

attachment and trophoblast outgrowth were compared respectively.Results:The rate of zona-hatching

attachment and trophoblast outgrowth with GRC-1 were 89.68%

86.51 %和84.92%

respectively.These were significantly higher than those of the control group ( P <0.01 ).Conclusion:GRC-1 growth fluid is a kind of good culture medium for growth and development of mouse embryo in-vitro.

关键词

肾癌细胞株GRC-1组织培养小鼠胚胎

Keywords

GRC-1Culturemouseembryo

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Establishment of the LAMP rapid detection method for mouse Sendai virus

Experimental study of the effect of murine submandibular gland conditioned media on hematopoietic stem cell and bone marrow cell proliferation

A study on the relationship between the infection of human cytomegalovirus(HCMV) and the variation of kinetochore

Expression of leukaemia inhibitory factor from endometria of estrus cycle in mouse

Effects of mouse blastocysts on invasive,adhesive and mobile capacity of human endometrial carcinoma cells in vitro

Related Author

Lai Guoqi

Tan Yi

Wang Sheng

Pi Guanglu

Yan Lei

Wang Lei

Wang Huijuan

祝彼得

Related Institution

Experimental Animal Center,Chongqing Medical University

Animal Health Surveillance Institute of Yuzhong District in Chongqing

成都中医药大学

Department of Histology and Embryology, College of Basic Medical Sciences

重庆医科大学计划生育系遗传优生教研室

⁰