HPLC determination of adenosine phosphates in mouse myocardium

CHEN Jianbin; et al

doi:10.13406/j.cnki.cyxb.2003.04.023

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HPLC determination of adenosine phosphates in mouse myocardium

更新时间：2024-01-17

- HPLC determination of adenosine phosphates in mouse myocardium
- Journal of Chongqing Medical University Issue 4, Pages: 485-486(2003)
- 作者机构：
  
  1. 重庆医科大学临床学院内科
  2. 重庆医科大学临床学院临床药理研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.04.023
  CLC： O657.72
- Published：2003，
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[1]陈建斌,饶邦复,周远大,何海霞.高效液相色谱法测定心肌腺苷酸含量[J].重庆医科大学学报,2003(04):485-486+490.

CHEN Jianbin, et al. HPLC determination of adenosine phosphates in mouse myocardium. [J]. Journal of Chongqing Medical University (4):485-486(2003)
[1]陈建斌,饶邦复,周远大,何海霞.高效液相色谱法测定心肌腺苷酸含量[J].重庆医科大学学报,2003(04):485-486+490. DOI： 10.13406/j.cnki.cyxb.2003.04.023.

CHEN Jianbin, et al. HPLC determination of adenosine phosphates in mouse myocardium. [J]. Journal of Chongqing Medical University (4):485-486(2003) DOI： 10.13406/j.cnki.cyxb.2003.04.023.

摘要

目的 :用改良高效液相色谱法测定小鼠心肌腺苷酸含量。方法 :ODSC18柱

检测波长 2 5 4nm。甲醇 - 5 0mmol/L磷酸盐（1∶99）缓冲液（pH6 .0 7）为流动相。结果 :心肌ATP、ADP和AMP平均回收率分别为 10 1.1%± 9.3%、94 .8%± 10 .6 %及 98.3%± 5 .7% ;日内RSD分别为 3.5 %、6 .9%和 4 .8% ;日间RSD分别为 6 .4 %、8.6 %及 8.2 %。结论 :该方法简便、快速、灵敏。采用该法发现病毒性心肌炎小鼠心肌ATP、ADP和AMP含量降低。

Abstract

Objective:A method for the quantitative determination of adenosine phosphates of mouse myocardium by HPLC is described.Methods:A ODS C 18 column and a mobile phase of methol-50mmol·L -1 phosphate (1∶99)buffer solution (pH 6.07)

with UV detector at 254nm were used.Results:The average recovery rates of ATP

ADP and AMP were 101.7%±9.3%

94.8%±10.6% and 98.3%±5.7% respectively;the RSDs of within-day were 3.5%

6.8% and 4.8%

and the RSDs of between-days were 6.4%

8.6% and 8.2%

respectively.Conclusion:The method is simple

rapid and senstive and can be used to analyse the adenosine phosphates in myocardium.The myocardial contents of ATP

ADP as well as AMP decline notably in mice with viral myocarditis.

关键词

高效液相色谱法腺苷酸

Keywords

high performance liquid chromatography(HPLC)Adenosine phosphate

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