An experimental study on mesenchymal stem cells  differentiated into cardiomyocytes in vivo

LU Tiewei; et al

doi:10.13406/j.cnki.cyxb.2003.04.017

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An experimental study on mesenchymal stem cells differentiated into cardiomyocytes in vivo

更新时间：2024-01-17

- An experimental study on mesenchymal stem cells differentiated into cardiomyocytes in vivo
- Journal of Chongqing Medical University Issue 4, Pages: 463-466(2003)
- 作者机构：
  
  1. 重庆医科大学儿科学院心内科
  2. 重庆医科大学儿科学院儿科研究所
  3. 重庆医科大学儿科学院心内科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.04.017
  CLC： R329
- Published：2003，
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[1]吕铁伟,田杰,朱静,钱永如.骨髓间充质干细胞体内诱导分化为心肌细胞的初步实验观察[J].重庆医科大学学报,2003(04):463-466.

LU Tiewei, et al. An experimental study on mesenchymal stem cells differentiated into cardiomyocytes in vivo. [J]. Journal of Chongqing Medical University (4):463-466(2003)
[1]吕铁伟,田杰,朱静,钱永如.骨髓间充质干细胞体内诱导分化为心肌细胞的初步实验观察[J].重庆医科大学学报,2003(04):463-466. DOI： 10.13406/j.cnki.cyxb.2003.04.017.

LU Tiewei, et al. An experimental study on mesenchymal stem cells differentiated into cardiomyocytes in vivo. [J]. Journal of Chongqing Medical University (4):463-466(2003) DOI： 10.13406/j.cnki.cyxb.2003.04.017.

摘要

目的 :初步观察MSCs体内诱导分化为心肌细胞的能力。方法 :从大鼠双侧股骨骨髓获得MSCs

在体外纯化、扩增后

DAPI进行细胞标记

然后注射到急性心肌梗塞模型鼠和正常大鼠的心肌组织内

饲养 2～ 4周后

处死动物在注射点获取心肌标本

初步采用HE染色和电镜观察形态学的方法对分化的心肌细胞进行鉴定

并用免疫组化法从组织学上对转化心肌细胞进行心肌特异性抗原的检测。结果 :MSC进行DAPI的标记效率高

电镜观察与宿主心肌细胞间形成了闰盘

组化检测有心肌特异性抗原的出现。结论 :在宿主心肌组织内

MSCs具有分化为心肌细胞的能力。

Abstract

Objective:To investigate the ability of mesenchymal stem cells differentiated into cardiomyocytes in vivo.Methods:MSCs isolated form rats bilateral tibias were purified

expanded in vitro culture

labeled with DAPI

then injected into the myocardium of the recipients(including acute myocardial infraction and normal rats).The hearts were harvested 2～4 weeks after implantation.Morphology of the differentiated cardiomyocytes was identified using HE stain and electron microscopy.Cardiac-specific antigen was detected using immunohistochemistry.Results:Viable cells labeled with DAPI can be identified in host myocardium at all time points after implantation.Implanted MSCs showed the growth potential in myocardial environments

after 4 weeks

donor cells derived from MSCs demonstrated myogenic differentiation with the expressing of myosin heavy chain.Donor cells demonstrated cardiomyocytes phenotypes with the expressing of cardiac-specific antigen Cx43.Meanwhile

electron microscopy examination demonstrated the existence of intercalated disks between donor cells and host cells.Conclusion:MSCs can differentiate into cardiomyoytes in host myocardial microenvironments.

关键词

骨髓间充质干细胞（MSCs）心肌细胞诱导分化

Keywords

Mesenchymal stem cellscardiomyocytesdifferentiation

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