NF-κ B inhibitor-PDTC enhance TNF-α induced cytotoxicity  in breast cancer cells

TU Gang; et al

doi:10.13406/j.cnki.cyxb.2003.04.014

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NF-κ B inhibitor-PDTC enhance TNF-α induced cytotoxicity in breast cancer cells

更新时间：2024-01-17

- NF-κ B inhibitor-PDTC enhance TNF-α induced cytotoxicity in breast cancer cells
- Journal of Chongqing Medical University Issue 4, Pages: 454-456(2003)
- 作者机构：
  
  1. 重庆医科大学临床学院普外科
  2. 重庆医科大学临床学院普外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.04.014
  CLC： R737.9
- Published：2003，
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[1]涂刚,姚榛祥.NF-κB抑制剂PDTC增敏TNF-α对乳腺癌细胞的毒性作用[J].重庆医科大学学报,2003(04):454-456.

TU Gang, et al. NF-κ B inhibitor-PDTC enhance TNF-α induced cytotoxicity in breast cancer cells. [J]. Journal of Chongqing Medical University (4):454-456(2003)
[1]涂刚,姚榛祥.NF-κB抑制剂PDTC增敏TNF-α对乳腺癌细胞的毒性作用[J].重庆医科大学学报,2003(04):454-456. DOI： 10.13406/j.cnki.cyxb.2003.04.014.

TU Gang, et al. NF-κ B inhibitor-PDTC enhance TNF-α induced cytotoxicity in breast cancer cells. [J]. Journal of Chongqing Medical University (4):454-456(2003) DOI： 10.13406/j.cnki.cyxb.2003.04.014.

摘要

目的 :探讨核因子κB（nuclearfactorkappaB）活化抑制剂二硫代氨基甲酸吡咯烷（pyrro1idinedithiocarbamate

PDTC）对肿瘤坏死因子α（tumornecrosisfactorα

TNF -α）细胞毒性作用的影响。方法 :用MTT法观察TNF -α、PDTC及二者联合作用对乳腺癌细胞系MCF - 7、MDA -MB - 4 35s体外生长的影响。将MDA -MB - 4 35s细胞接种于雌性BALB/c纯系小鼠肾包膜下复制出移植乳腺癌动物模型

观察TNF -α、PDTC及联合作用对体内移植肿瘤生长的影响。结果 :单用TNF -α（小于或等于 2 0 0 0U/ml）或PDTC均不影响两种乳腺癌细胞的体外生长

但是先PDTC（5 0 μmol）作用 1h

再应用TNF -α（2 0 0 0U/ml）即可明显地抑制乳腺癌细胞的生长。动物实验中治疗组应用TNF -α（7.5× 10 6U/kg/次

2次 /日

ip） +PDTC（5 0mg/kg/次

2次 /日

ip）

肿瘤平均体积为 1.0 8± 0 .32mm3

明显小于对照组平均瘤体 11.6 7± 0 .5 3mm3 （P <0 .0 1）

单用TNF -α组（10 .96± 1.0 4mm3 ）及PDTC组（11.72± 0 .73mm3 ）与对照组比较无显著差异（P >0 .0 5 ）。结论 :NP -κB抑制剂PDTC能够显著增敏TNF -α对乳腺癌细胞的毒性作用

抑制乳腺癌细胞的生长。

Abstract

Objective:To evaluate the effect of NF-κ B inhibitor-PDTC on TNF-α induced cytotoxicity in breast cancer cells.Methods:The cell survivals of breast cancer cell lines MCF-7 and MDA-MB-435s were measured by MTT assay in vitro.MDA-MB-435s cells were implanted into BALB/C mice. Animal tumor models were treated with TNF-α

PDTC and TNF-α plus PDTC.Results:The growth of two breast cancer cell lines was not inhibited by either TNF-α(2000U/mi or less)or PDTC.However

both breast cancer cells treated with TNF-α(2000U/mi)plus PDTC(50μ mol)showed significant growth inhibition as compared with controls. In vivo

the growth of implanted tumor was significantly reduced in volume in mice treated with TNF-α(7.5×10 6U/kg

bid) plus PDTC(50mg/kg

bid) as compared with controls( P <0.01).Tumor growth was not inhibited by either TNF-α or PDTC treatment.Conclusion:NF-κ B inhibitor-PDTC evidently enhance TNF-α in inducing cytotoxicity in breast cancer cells

and thus inhibit the growth of cells.

关键词

乳腺癌肿瘤坏死因子α核因子κB

Keywords

breast cancerTumor necrosis factor-αNuclear factor kappa B

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