Expression of Toll-like receptor 4 in kupffer cells  in experimental alcohol-induced liver disease

ZUO Guoqing; et al

doi:10.13406/j.cnki.cyxb.2003.04.011

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Expression of Toll-like receptor 4 in kupffer cells in experimental alcohol-induced liver disease

更新时间：2024-01-17

- Expression of Toll-like receptor 4 in kupffer cells in experimental alcohol-induced liver disease
- Journal of Chongqing Medical University Issue 4, Pages: 444-447(2003)
- 作者机构：
  
  1. 重庆医科大学第二临床学院消化内科
  2. 重庆医科大学第二临床学院肝胆外科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.04.011
  CLC： R575
- Published：2003，
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[1]左国庆,龚建平,何松,刘长安.实验性酒精性肝病时Kupffer细胞Toll样受体4的表达[J].重庆医科大学学报,2003(04):444-447.

ZUO Guoqing, et al. Expression of Toll-like receptor 4 in kupffer cells in experimental alcohol-induced liver disease. [J]. Journal of Chongqing Medical University (4):444-447(2003)
[1]左国庆,龚建平,何松,刘长安.实验性酒精性肝病时Kupffer细胞Toll样受体4的表达[J].重庆医科大学学报,2003(04):444-447. DOI： 10.13406/j.cnki.cyxb.2003.04.011.

ZUO Guoqing, et al. Expression of Toll-like receptor 4 in kupffer cells in experimental alcohol-induced liver disease. [J]. Journal of Chongqing Medical University (4):444-447(2003) DOI： 10.13406/j.cnki.cyxb.2003.04.011.

摘要

目的 :观察酒精性肝病大鼠Kupffer细胞（KCs）Toll样受体（TLR） 4蛋白合成和基因表达及其在肝损害中的作用。方法 :2 8只Wistar大鼠随机分为乙醇喂养组（E组）和葡萄糖喂养组（C组）。用激光共聚焦显微镜测定KCs膜上TLR4蛋白的合成 ;用逆转录多聚酶联反应（RT -PCR）测定KCs中TLR4mRNA的表达 ;用鲎试剂基质偶氮显色法测定门静脉血中内毒素含量

并观察肝脏形态学变化。结果 :E组KCs膜TLR4蛋白表达较C组显著增强 ;E组TLR4mRNA的表达也显著高于C组（P <0 .0 1） ;E组血浆内毒素浓度明显高于C组（P <0 .0 1） ;E组肝组织发生显著的病理变化。结论 :乙醇能诱导大鼠肝脏KCs合成TLR4蛋白及表达TLR4基因

TLR4在乙醇所致的肝损害中可能起作用。

Abstract

Objective:To observe the synthesis of Toll-like receptor (TLR)4 protein and its mRNA expression in Kupffer cells (KCs) and evaluate the role of TLR 4 in the liver injury in the rats with alcohol-induced liver disease (ALD).Methods:Twenty eight Wistar rats were divided into two groups: ethanol-fed group(group E) and control group (group C). Group E were fed with ethanol (dose of 5～12g/kg/d) and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 wk and 8 wk. KCs were isolated and incubated.TLR 4 protein in KCs was determined by laser scanning confocal microscopy (LSCM).TLR 4 mRNA in KCs was determined by the reverse transcription polymerase chain reaction (RT-PCR).Plasma endotoxin levels were measured using the Limulus Amebocyte Lysate test kit.The liver pathology was observed under light and electronic microscopy.Results:The fluorescence intensity of TLR 4 protein in KCs by LSCM was stronger in group E than those in group C. Ethanol administration led to a significant increase in TLR 4 mRNA expression compared with group C ( P< 0.01).Plasma endotoxin levels increased significantly in group E ( 129±21)ng/L and (187±35 )ng/L at than in control rats (48±9)ng/L and (53±11)ng/L

respectively at 4 wk and 8 wk ( P <0.01). In liver section from group E

there was marked pathological changes including steatosis

cell infiltration and necrosis. None of the rats in group C developed marked pathological changes in liver. Conclusion: Ethanol administration can markedly lead to the synthesis of TLR 4 protein and its gene expression in KCs

and TLR 4 may play a role in the development of alcohol-induced liver injury.

关键词

Toll样受体酒精性肝病Kupffer细胞

Keywords

Toll-like receptorAlcohol-induced liver diseaseKupffer cells

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