Experimental study on expressing GM-CSF in human bone marrow  stromal cells by total saponins of Panax ginseng (TSPG)

CHEN Di; et al

doi:10.13406/j.cnki.cyxb.2003.04.001

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Experimental study on expressing GM-CSF in human bone marrow stromal cells by total saponins of Panax ginseng (TSPG)

更新时间：2024-01-17

- Experimental study on expressing GM-CSF in human bone marrow stromal cells by total saponins of Panax ginseng (TSPG)
- Journal of Chongqing Medical University Issue 4, Pages: 405-408(2003)
- 作者机构：
  
  1. 重庆医科大学基础医学院生理学教研室
  2. 重庆医科大学基础医学院组织胚胎学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.04.001
  CLC： R285.5
- Published：2003，
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[1]陈笛,王莎莉,王亚平,王璐,姜蓉.人参总皂甙诱导人骨髓基质细胞表达GM-CSF的实验研究[J].重庆医科大学学报,2003(04):405-408.

CHEN Di, et al. Experimental study on expressing GM-CSF in human bone marrow stromal cells by total saponins of Panax ginseng (TSPG). [J]. Journal of Chongqing Medical University (4):405-408(2003)
[1]陈笛,王莎莉,王亚平,王璐,姜蓉.人参总皂甙诱导人骨髓基质细胞表达GM-CSF的实验研究[J].重庆医科大学学报,2003(04):405-408. DOI： 10.13406/j.cnki.cyxb.2003.04.001.

CHEN Di, et al. Experimental study on expressing GM-CSF in human bone marrow stromal cells by total saponins of Panax ginseng (TSPG). [J]. Journal of Chongqing Medical University (4):405-408(2003) DOI： 10.13406/j.cnki.cyxb.2003.04.001.

摘要

目的 :研究人参总皂甙（TSPG）对人骨髓基质细胞表达GM -CSF的影响及其与人粒单系血细胞发生的关系。方法 :采用造血祖细胞与骨髓基质细胞体外培养、造血生长因子生物学活性检测、免疫细胞化学和核酸分子原位杂交等技术。结果 :TSPG能显著刺激粒单系造血祖细胞（CFU -GM ）增殖 ;经TSPG诱导制备的骨髓基质细胞条件培养液富含GM -CSF样活性 ;TSPG能显著促进骨髓基质细胞的GM -CSF蛋白和mRNA表达。结论 :TSPG可能通过直接和 /或间接途径促进造血诱导微环境中的骨髓基质细胞合成和分泌GM -CSF

进而促进CFU -GM的增殖分化。

Abstract

Objective: To study the relationship between effect of total saponins of Panax ginseng(TSPG) on expression of GM-CSF in human bone marrow stromal cells and modulation of human granulocytopoiesis and monocytopoiesis.Methods:The techniques of culture of hematopoietic progenitor cells and human bone marrow stromal cells in vitro

immunocytochemistry

nucleic acid in situ hybridization were used.Results:TSPG could markedly promote the colony formation of CFU-GM; human bone marrow stromal cells conditioned media prepared with TSPG could significantly enhance the proliferation and differentiation of CFU-GM

and expression of GM-CSF in protein and mRNA level in human bone marrow stromal cells induced by TSPG were much higher than those of the control group.Conclusion:TSPG may directly and/or indirectly promote stromal cells in hematopoietic inductive microenvironment to produce GM-CSF

which further prowote the proliferation and differentiation of human CFU-GM.

关键词

人参总皂甙粒单系造血祖细胞粒-巨噬细胞集落刺激因子

Keywords

Total saponins of panax ginsengCFU-GMGM-CSF

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Department of Histology and Embryology,College of Basic Medical Sciences， Chongqing Medical University

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