An experiment study on apoptosis in alcohol-induced early avascular necrosis of the femoral head

GUO Kunliang; et al

doi:10.13406/j.cnki.cyxb.2003.03.017

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An experiment study on apoptosis in alcohol-induced early avascular necrosis of the femoral head

更新时间：2024-01-17

- An experiment study on apoptosis in alcohol-induced early avascular necrosis of the femoral head
- Journal of Chongqing Medical University Issue 3, Pages: 313-315(2003)
- 作者机构：
  
  1. 重庆医科大学临床学院骨科
  2. 重庆医科大学临床学院骨科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.03.017
  CLC： R681.8
- Published：2003，
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[1]郭坤亮,安洪,蒋电明,邓忠良,刘传康,李宏宇.早期酒精性股骨头缺血坏死骨细胞凋亡的实验研究[J].重庆医科大学学报,2003(03):313-315.

GUO Kunliang, et al. An experiment study on apoptosis in alcohol-induced early avascular necrosis of the femoral head. [J]. Journal of Chongqing Medical University (3):313-315(2003)
[1]郭坤亮,安洪,蒋电明,邓忠良,刘传康,李宏宇.早期酒精性股骨头缺血坏死骨细胞凋亡的实验研究[J].重庆医科大学学报,2003(03):313-315. DOI： 10.13406/j.cnki.cyxb.2003.03.017.

GUO Kunliang, et al. An experiment study on apoptosis in alcohol-induced early avascular necrosis of the femoral head. [J]. Journal of Chongqing Medical University (3):313-315(2003) DOI： 10.13406/j.cnki.cyxb.2003.03.017.

摘要

目的 :探讨细胞凋亡在早期酒精性股骨头缺血坏死中的意义。方法 :采用灌胃法

给予实验组家兔含乙醇 5 0 %的烈性白酒 8mL（kg·d） 1～ 6月

对照组给予等量生理盐水 ;观察股骨组织学变化

用末端原位标识（TUNEL）法检测骨细胞凋亡。结果 :2个月时实验组凋亡细胞数（10 7.6 0‰± 2 5 .87‰ ）明显高于对照组（P <0 .0 1）

并随着时间推移

凋亡细胞逐渐增多 ;与对照组（10 .6 9%± 3.11%

11.2 4 %± 2 .6 4 % ）相比

3、6个月时实验组空骨陷窝百分比（16 .6 1%± 3.6 0 %

2 7.93%± 3.0 6 % ）明显增加（P <0 .0 5

P <0 .0 1）。透射电镜发现部分胃细胞核膜完整、染色质浓集、电子密度增加的凋亡特征。结论 :细胞凋亡在早期酒精性股骨头缺血坏死中起重要作用。

Abstract

Objective:To explore the significance of osteocyte apoptosis in alcohol-induced avascular necrosis of the femoral head.Methods:The experimental group was administered the wine containing 50% of alcohol with dosage of 8 mL/(kg.d) and isodose NS as controls by pouring into stomach with a plastic tube for 1 to 6 months

to observe the changes of histology in the femoral head and to study osteocytes apoptosis by means of TdT-mediated dUTP Nick End Labeling (TUNEL).Results:The number of apoptosis in the experimental group (107.60‰±25.87‰) was significantly higher than the control ( P <0.01) in second month.With the passing of the time

osteocytes apoptosis progressively increased.In third and sixth month

the percentage of empty osteocyte lacunae in the experimental group was increased remarkably to 16.61%±3.60% and 27.93%±3.06%

respectively

compared with the controls(10.69%±3.11%

11.24%±2.64%)( P <0.05

P <0.01).The transmissible electron microscope study of the osteocyte apoptosis showed that characteristics included intact nuclear membrane

condensed chromatin and increased electron dense.Conclusion:Osteocytes apoptosis may play a key role in the process of alcohol-induced early avascular necrosis of the femoral head.

关键词

细胞凋亡酒精股骨头坏死

Keywords

apoptosisAlcoholFemoral headnecrosis

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