The impact of rh-LIF on HL-60 cell line

DU Bing; et al

doi:10.13406/j.cnki.cyxb.2003.03.005

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The impact of rh-LIF on HL-60 cell line

更新时间：2024-01-17

- The impact of rh-LIF on HL-60 cell line
- Journal of Chongqing Medical University Issue 3, Pages: 270-272(2003)
- 作者机构：
  
  1. 重庆医科大学基础医学院微生物学教研室
  2. 川北医学院微生物学教研室,重庆,400016
  3. ,南充,637000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.03.005
  CLC： R733.7
- Published：2003，
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[1]杜冰,朱道银,唐恩洁.重组人白血病抑制因子对HL-60细胞的作用及机制研究[J].重庆医科大学学报,2003(03):270-272.

DU Bing, et al. The impact of rh-LIF on HL-60 cell line. [J]. Journal of Chongqing Medical University (3):270-272(2003)
[1]杜冰,朱道银,唐恩洁.重组人白血病抑制因子对HL-60细胞的作用及机制研究[J].重庆医科大学学报,2003(03):270-272. DOI： 10.13406/j.cnki.cyxb.2003.03.005.

DU Bing, et al. The impact of rh-LIF on HL-60 cell line. [J]. Journal of Chongqing Medical University (3):270-272(2003) DOI： 10.13406/j.cnki.cyxb.2003.03.005.

摘要

目的 :观察rh -LIF对HL - 6 0细胞生长与分化的影响及其可能的作用机制。方法 :不同剂量的rh -LIF作用HL -6 0细胞 3- 6天

观察细胞生长

流式细胞术分析细胞周期

用免疫组化法检测P5 3

P2 1蛋白表达的变化

原位杂交法检测P5 3mRNA表达水平的改变。结果 :rh -LIF可诱导HL - 6 0细胞向单核 /巨噬细胞分化

抑制HL - 6 0细胞的增殖

使细胞停滞于G1期 :P5 3

P2 1蛋白和P5 3mRNA表达明显增强。结论 :rh -LIF对HL - 6 0细胞具增殖抑制和诱导分化作用

其作用机制可能与G1期阻滞及P5 3

P2 1表达增高有关。

Abstract

Objective:To investigate the effect and mechanism of rh-LIF on proliferation and differentiation of HL-60 cell line.Methods:HL-60 cells were treated with different dosage of rh-LIF for 3 to 6 days

cell cycle was analysed by flow cytometry

expression of P53 and P21 protein were observed by immunohistochemistry and P53 mRNA were assayed by in situ hybridization.Results:HL-60 cell line could differentiate into monocyte and macrophage cell line in the presence of rh-LIF.The proliferation of HL-60 cells were inhibited in the presence of rh-LIF

the cells stagnated at G 1 stage

while expression of P53

P21 protein and P53 mRNA were increased obviously.Conclusion:The effects of rh-LIF on the proliferation and differentiation of HL-60 cells may be associated with the increase of P53

P21 protein and P53mRNA.

关键词

重组人白血病抑制因子诱导分化生长抑制

Keywords

rh-LIFdifferentiationGrowth inhibition

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