Pattern of protein kinase C isoforms expression  in retinal vasculature of diabetic rats

ZHOU Bo; et al

doi:10.13406/j.cnki.cyxb.2003.03.004

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Pattern of protein kinase C isoforms expression in retinal vasculature of diabetic rats

更新时间：2024-01-17

- Pattern of protein kinase C isoforms expression in retinal vasculature of diabetic rats
- Journal of Chongqing Medical University Issue 3, Pages: 267-269(2003)
- 作者机构：
  
  1. 重庆医科大学临床学院内分泌科
  2. 重庆医科大学基础医学院生化教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.03.004
  CLC： R587.1
- Published：2003，
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[1]周波,汪恕萍,蒋涛,曾昭淳.糖尿病大鼠视网膜血管蛋白激酶C同工酶表达模式[J].重庆医科大学学报,2003(03):267-269+306.

ZHOU Bo, et al. Pattern of protein kinase C isoforms expression in retinal vasculature of diabetic rats. [J]. Journal of Chongqing Medical University (3):267-269(2003)
[1]周波,汪恕萍,蒋涛,曾昭淳.糖尿病大鼠视网膜血管蛋白激酶C同工酶表达模式[J].重庆医科大学学报,2003(03):267-269+306. DOI： 10.13406/j.cnki.cyxb.2003.03.004.

ZHOU Bo, et al. Pattern of protein kinase C isoforms expression in retinal vasculature of diabetic rats. [J]. Journal of Chongqing Medical University (3):267-269(2003) DOI： 10.13406/j.cnki.cyxb.2003.03.004.

摘要

目的 :探讨糖尿病大鼠视网膜血管蛋白激酶C（PKC）活性变化及其同工酶表达特性。方法 :采用渗透休克法获取正常及链脲佐菌素诱导的糖尿病大鼠视网膜血管。原位PKC催化活性分析法测定两组大鼠视网膜血管PKC活性

α、β、γ、δ、ε、ζ-PKC同工酶表达水平则采用Westernblot法。结果 :病程 2月时糖尿病大鼠视网膜血管PKC活性为 346± 18.1PmolesPimin-1·mgprotein-1（单位下同）较正常对照组 131± 10 .5显著为高（P <0 .0 5 ）。α、β、δ、ζ -PKC分布于正常和糖尿病大鼠血管细胞浆、膜部

而γ、ε则无信号

α、β、ζ同工酶在糖尿病大鼠视网膜血管膜部较正常对照大鼠显著为高（P <0 .0 5 ）

尤以α、β为著

光密度（OD）值分别为对照的 2 82 %± 32 %、340 %± 2 6 %、187%± 14 %。而糖尿病大鼠视网膜血管浆部α、β、ζ -PKC同工酶表达则较对照组显著为低（P <0 .0 5 ）

其OD值分别为对照的 71%± 11%、6 3%± 8%、84 %± 6 %。但δ -PKC浆部则较对照组显著增高

其OD值为正常对照之 2 0 8%± 13%

但膜部δ -PKC与正常对照相比较无显著性差异。结论 :糖尿病视网膜病变（DR）靶组织———视网膜血管中PKC显著激活

呈α、β、ζ同工酶从胞浆至胞膜

而δ -PKC则从胞膜至胞浆移位激活特征。

Abstract

Objective:To clarify the characteristics of protein kinase C(PKC)activity and its isoforms expression pattern in retinal vessels in diabetic rats.Methods:Osmotic shock method was used to isolate the retinal vessels of rats;in situ PKC catalytic activity was determined after 2 mo follow-up;α?β?γ?δ?ε?ζ-PKC in the membrane and cytosolic fraction were determined by western blot.Results:Diabetes result in>180% elevation of PKC activity in the retinal vasculature.α?β?δ?ζ?except γ?ε distributed in the membrane and cytosolic fraction in retinal vessels in normal or diabetic rats

the expression of α?β?ζ-PKC significantly increase in the membrane pool OD Value 282%±32%?340%±26%?187%±14% respectively

but α?β?ζ-PKC expression significantly decrease in cytosolic pool in retinal vessels of diabetic rats compared with nondiabetic rats

OD value 71%±11%?63%±8%?84%±6%

respectively

however δ isoenzym expression in cytosolic pool significantly increase 208%±6%

compared with normal rats.Conclusion:Diabetes can induce the translocation of α?β?ζ-PKC from cytosol to membrane pool

but δ isoform translocated from membrane to cytosol pool in retinal vessels of diabetic rats.

关键词

蛋白激酶C同工酶血管视网膜糖尿病

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