Experimental study of the effect of Angelica polysaccharide on proliferation and differentiation of human CFU-GM and HL-60 cells

WANG Shali; et al

doi:10.13406/j.cnki.cyxb.2003.03.001

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Experimental study of the effect of Angelica polysaccharide on proliferation and differentiation of human CFU-GM and HL-60 cells

更新时间：2024-01-17

- Experimental study of the effect of Angelica polysaccharide on proliferation and differentiation of human CFU-GM and HL-60 cells
- Journal of Chongqing Medical University Issue 3, Pages: 257-260(2003)
- 作者机构：
  
  1. 重庆医科大学生理学教研室
  2. 重庆医科大学组织胚胎学教研室,重庆,400016
  3. 重庆医科大学临床学院血液内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.03.001
  CLC： R733.7
- Published：2003，
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[1]王莎莉,王亚平,戴勤,姜蓉.当归多糖对人粒单系祖细胞和粒系白血病细胞株增殖分化的实验研究[J].重庆医科大学学报,2003(03):257-260.

WANG Shali, et al. Experimental study of the effect of Angelica polysaccharide on proliferation and differentiation of human CFU-GM and HL-60 cells. [J]. Journal of Chongqing Medical University (3):257-260(2003)
[1]王莎莉,王亚平,戴勤,姜蓉.当归多糖对人粒单系祖细胞和粒系白血病细胞株增殖分化的实验研究[J].重庆医科大学学报,2003(03):257-260. DOI： 10.13406/j.cnki.cyxb.2003.03.001.

WANG Shali, et al. Experimental study of the effect of Angelica polysaccharide on proliferation and differentiation of human CFU-GM and HL-60 cells. [J]. Journal of Chongqing Medical University (3):257-260(2003) DOI： 10.13406/j.cnki.cyxb.2003.03.001.

摘要

目的 :研究当归多糖（APS）对人粒单系造血祖细胞（CFU -GM ）和急性早幼粒白血病细胞株（HL - 6 0 ）增殖分化的影响。方法 :采用造血细胞体外培养

形态学观察

流式细胞术和免疫细胞化学等实验血液学技术。结果 :APS对正常CFU -GM的体外增殖有显著刺激作用 ;而对HL - 6 0细胞的增殖却有明显的抑制作用 ;APS可能通过阻止HL - 6 0细胞从静止期进入增殖周期

抑制DNA的合成等途径来抑制细胞增殖 ;APS可能诱导HL - 6 0细胞发生凋亡。结论 :APS有可能成为既能促进正常造血

又能抑制白血病等肿瘤细胞增殖

并诱导其凋亡或分化的天然诱导剂。

Abstract

Objective:To study modern biological mechanism for hematonic and antitumor of Angelica Sinensis (oliv) Diels.Methods:By using the techniques of hematopoietic cells culture in vitro

morphological observation

flow cytometry and immunocytochemistry

the effect of Angelica polysaccharide (APS) on proliferation and differentiation of human CFU-GM and promyelocytic leukemia cell line (HL-60) was studied.Results:APS could markedly promote the colony formation of CFU-GM and could significantly inhibit the proliferation of HL-60 in vitro.The inhibitory effect is concerned with the concentration of APS.APS may prevent HL-60 from the Go phase entering the S/G2+M phase

thus prohibit the synthesis of DNA.APS may also promote HL-60 entering the process of apoptosis.Conclusion:APS may not only promote normal hematopoiesis

but also inhibit the proliferation of leukemia cell and it can be a natural inducer for therapy of malignant tumor.

关键词

当归多糖粒单系祖细胞粒系白血病细胞株人

Keywords

Angelica polysaccharideCFU-GMHL-60Human

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