The effect of sperm washing on hepatitis B virus concentration in semen

ZHOU Qin; YAO Zhenwei

doi:10.13406/j.cnki.cyxb.2003.02.022

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The effect of sperm washing on hepatitis B virus concentration in semen

更新时间：2024-01-17

- The effect of sperm washing on hepatitis B virus concentration in semen
- Journal of Chongqing Medical University Issue 2, Pages: 189-191(2003)
- 作者机构：
  
  1. 重庆医科大学临床学院妇产科
  2. 重庆医科大学临床学院妇产科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.02.022
  CLC： R446.5
- Published：2003，
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[1]周勤,姚珍薇.精子洗涤对精液中HBV DNA含量的影响[J].重庆医科大学学报,2003(02):189-191+197.

ZHOU Qin, YAO Zhenwei. The effect of sperm washing on hepatitis B virus concentration in semen. [J]. Journal of Chongqing Medical University (2):189-191(2003)
[1]周勤,姚珍薇.精子洗涤对精液中HBV DNA含量的影响[J].重庆医科大学学报,2003(02):189-191+197. DOI： 10.13406/j.cnki.cyxb.2003.02.022.

ZHOU Qin, YAO Zhenwei. The effect of sperm washing on hepatitis B virus concentration in semen. [J]. Journal of Chongqing Medical University (2):189-191(2003) DOI： 10.13406/j.cnki.cyxb.2003.02.022.

摘要

目的 :检查HBV感染者精液是否存在乙型肝炎病毒（HBV）

探讨精子洗涤是否能去除其中的HBVDNA。方法 :用实时荧光定量PCR（FQ -PCR）法检测 5 7名血清HBsAg阳性患者精液中HBVDNA

采用Percoll非连续梯度离心及上游法分离其中 2 1份精液

并对洗涤后标本进行HBVDNA检测。结果 :6 2份精液标本中有 15份HBVDNA阳性

阳性率为2 4 .19% ;2 1份用于洗涤的精液标本中

有 7份HBVDNA阳性

洗涤后仍有 6份标本阳性。结论 :部分HBV感染者精液具有传染性

精子洗涤不能完全去除精液中的乙肝病毒。将HBV感染者的精液用于人工授精或体外受精应慎重。

Abstract

Objective: To detect hepatitis B virus (HBV) in semen and to find if sperm washing removes HBV DNA. Methods: Amplification by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect viral DNA in semen samples of 57 HBsAg-seropositive patients.Twenty-one semen samples were washed

and spermatozoa were separated by discontinuous Percoll gradient centrifugation and the `swim-up’ method.The HBV DNA in post-washing samples was measured by FQ-PCR. Results: HBV DNA was detected in 15 of 62 semen samples

the positive rate was 24.19%.There were 7 positive cases in 21 samples before washing

the 6 cases except one were positive after washing. Conclusion: Part of semen of HBV infected patients is infective

and sperm washing does not remove HBV.It is cautious to use semen of the HBV infected patients in IVI or IVF.

关键词

乙型肝炎病毒精液精子洗涤

Keywords

hepatitis B virusSemenSperm washing

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Related Institution

Medical College of Wuhan University of Science and Technology

Department of Infectious Diseases， Puren Hospital Affiliated to Wuhan University of Science and Technology

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