Detection of ureaplasma urealyticum with two methods and anti-UU susceptibility test

XU Biao; et al

doi:10.13406/j.cnki.cyxb.2003.02.019

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Detection of ureaplasma urealyticum with two methods and anti-UU susceptibility test

更新时间：2024-01-17

- Detection of ureaplasma urealyticum with two methods and anti-UU susceptibility test
- Journal of Chongqing Medical University Issue 2, Pages: 181-182(2003)
- 作者机构：
  
  1. 重庆医科大学临床学院检验科
  2. 重庆医科大学临床学院检验科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.02.019
  CLC： R446.5
- Published：2003，
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[1]胥飚,曹何,余显书.解脲脲原体的检测及耐药性分析[J].重庆医科大学学报,2003(02):181-182+186.

XU Biao, et al. Detection of ureaplasma urealyticum with two methods and anti-UU susceptibility test. [J]. Journal of Chongqing Medical University (2):181-182(2003)
[1]胥飚,曹何,余显书.解脲脲原体的检测及耐药性分析[J].重庆医科大学学报,2003(02):181-182+186. DOI： 10.13406/j.cnki.cyxb.2003.02.019.

XU Biao, et al. Detection of ureaplasma urealyticum with two methods and anti-UU susceptibility test. [J]. Journal of Chongqing Medical University (2):181-182(2003) DOI： 10.13406/j.cnki.cyxb.2003.02.019.

摘要

目的 :探讨用聚合酶链反应 -微孔板杂交法（PCR -MPH）对解脲脲原体（UU）感染的检测

并分析药敏情况。方法 :将取自 4 96例泌尿生殖道感染患者的标本利用法国生物梅里埃公司的MycoplasmaIST培养基进行培养检测及药敏试验

对其中 2 12例标本同时进行PCR -MPH法检测。结果 :PCR -MPH法阳性检出率为 90 / 2 12 （42 .5 % ）

培养法为 78/ 2 12（36 .8% ） ;二者符合率为 90 .6 % ;PCR -MPH法的特异性与灵敏度分别 88.1%、94 .9%。 4 96例标本培养结果

女性阳性率10 1/ 2 2 6 （44 .7% ）

男性 6 4 / 2 70 （2 3.7% ）

二者有显著性差异（χ2 =2 4 .4 1

P <0 .0 1） ;药敏结果显示 :敏感率原始霉素 99.4 %、强力霉素 91.5 %、交沙霉素 89.7%、四环素 80 .0 %、红霉素 4 9.7%、氧氟沙星 2 5 .5 %。结论 :PCR -MPH作为一种快速、灵敏、特异的新方法

可在临床实验室诊断中推广应用 ;女性患者UU的检出率较男性高

应引起重视 ;合理使用抗生素

对治疗UU感染十分重要。

Abstract

Objective: To evaluate the detection of ureaplasma urealyticum(UU) with PCR-microplate hybridization (PCR-MPH) and culture

and analyze drug resistant activities. Methods: 496 samples were cultured and drug resistant activities analyzed by Bio-Merieux Mycoplasma-IST

and 212 samples of them were determined by PCR-MPH. Results: The positive rates of PCR-MPH and culture were determined to be 90/212(42.5%) and 78/212(36.8%)

respectively.The specificity and sensitivity of PCR-MPH were 88.1% and 99.4%

respectively.The positive rates of females and males with culture were 101/226(44.7%) and 64/270(23.7%)

respectively.There was significant difference between them(χ 2=24.41

P <0.01).The susceptibility rates of PRI

DOX

JOS

TET

ERY and OFL were 99.4%

91.5%

89.7%

80.0%

49.7% and 25.5%

respectively. Conclusion: PCR-MPH is rapid

sensitive

specific

and is of value in clinical diagnosis and therapy.Higher positive rate of the infected females should be paid attention to.It is very important to select antibiotics correctly for infections of the patients according to results of UU susceptibility tests.

关键词

解脲脲原体聚合酶链反应抗药性微生物

Keywords

Ureaplasma urealyticumPolymerase chain reactionDrug resistancemicrobial

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