Experimental and clinical study of in vitro chemosensitivity assay for laryngeal carcinoma and nasopharyngeal carcinoma by AgNOR methods

HU Guohua; et al

doi:10.13406/j.cnki.cyxb.2003.02.016

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Experimental and clinical study of in vitro chemosensitivity assay for laryngeal carcinoma and nasopharyngeal carcinoma by AgNOR methods

更新时间：2024-01-17

- Experimental and clinical study of in vitro chemosensitivity assay for laryngeal carcinoma and nasopharyngeal carcinoma by AgNOR methods
- Journal of Chongqing Medical University Issue 2, Pages: 172-174(2003)
- 作者机构：
  
  1. 重庆医科大学临床学院耳鼻咽喉科
  2. 重庆医科大学临床学院器官移植研究室
  3. 重庆医科大学临床学院耳鼻咽喉科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.02.016
  CLC： R739.65
- Published：2003，
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[1]胡国华,魏莲枝,孙余才,董蒲江,朱江.喉癌和鼻咽癌体外药敏试验AgNOR法的实验及临床研究[J].重庆医科大学学报,2003(02):172-174.

HU Guohua, et al. Experimental and clinical study of in vitro chemosensitivity assay for laryngeal carcinoma and nasopharyngeal carcinoma by AgNOR methods. [J]. Journal of Chongqing Medical University (2):172-174(2003)
[1]胡国华,魏莲枝,孙余才,董蒲江,朱江.喉癌和鼻咽癌体外药敏试验AgNOR法的实验及临床研究[J].重庆医科大学学报,2003(02):172-174. DOI： 10.13406/j.cnki.cyxb.2003.02.016.

HU Guohua, et al. Experimental and clinical study of in vitro chemosensitivity assay for laryngeal carcinoma and nasopharyngeal carcinoma by AgNOR methods. [J]. Journal of Chongqing Medical University (2):172-174(2003) DOI： 10.13406/j.cnki.cyxb.2003.02.016.

摘要

目的 :用嗜银蛋白检测技术（AgNOR）分析喉癌和鼻咽癌对 8种常用化疗药物的敏感性。方法 :从 5 6例喉癌和鼻咽癌患者获取新鲜肿瘤组织标本

采用单纯机械分离法制备单细胞悬液

加入抗癌药物后培养 36h

细胞悬液涂片、银染

计数银染颗粒后计算均值下降率 ;12例患者同期行化疗。结果 :5 1例肿瘤获得实验成功

可评价率为 91.1% ;AgNOR颗粒均值下降率从 0～ 6 3%

在 8种单一药物中

对DDP的敏感率最高

其它较为敏感的药物为VCR、5 -FU、PYM ;鼻咽癌对抗癌药物的敏感性显著高于喉癌对抗癌药物的敏感性 ;体外药敏与体内疗效的总符合率为 80 %。结论 :AgNOR技术简便易行

与临床相关性好

是一种有应用前景的体外药敏筛选方法。

Abstract

Objective: To evaluate in vitro chemosensitivity of laryngeal carcinoma and nasopharyngeal carcinoma to eight kinds of antineoplastic drugs by AgNOR technique. Methods: Fresh specimens of 56 cases were obtained from laryngeal carcinoma and nasopharyngeal carcinoma.We severed tumor tissue with simple mechanical separation method.After the acting of drugs

cell smear and argyrophilic staining

the mean inhibiting rate was calculated.10 cases were treated with chemotherapy at the same time. Results: 51 cases′specimens succeeded in the experiments

and the rate of success was 91.1%.The changes of mean inhibiting rate was from 0 to 63%.The chemosensitivity to DDP was the highest in eight kinds of antineoplastic drugs.Secondly

VCR

5-FU

and PYM were the highest.The sensitivity to drugs in laryngeal carcinoma was significantly higher than nasopharyngeal carcinoma.The coincidence between the experiment and clinic was 80%. Conclusion: AgNOR method is simple and convenient.In addition

it has clear correlation with the results of therapy.The method is useful for chemotherapy.

关键词

喉癌鼻咽癌体外药敏AgNOR

Keywords

laryngeal carcinomanasopharyngeal carcinomaIn vitro chemosensitivityAgNOR

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