The influence of antisense oligodeoxynucleotide targeting against telomerase   in the expression of cellular oncogene to nasopharyngeal carcinoma cells

CAO Qian; et al

doi:10.13406/j.cnki.cyxb.2003.02.013

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The influence of antisense oligodeoxynucleotide targeting against telomerase in the expression of cellular oncogene to nasopharyngeal carcinoma cells

更新时间：2024-01-17

- The influence of antisense oligodeoxynucleotide targeting against telomerase in the expression of cellular oncogene to nasopharyngeal carcinoma cells
- Journal of Chongqing Medical University Issue 2, Pages: 162-165(2003)
- 作者机构：
  
  1. 重庆医科大学第二临床学院耳鼻喉科
  2. 重庆医科大学第二临床学院耳鼻喉科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.02.013
  CLC： R739.63
- Published：2003，
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[1]曹前,吴家廉,骆文龙.反义寡核苷酸抑制端粒酶活性对鼻咽癌细胞癌基因表达的影响[J].重庆医科大学学报,2003(02):162-165.

CAO Qian, et al. The influence of antisense oligodeoxynucleotide targeting against telomerase in the expression of cellular oncogene to nasopharyngeal carcinoma cells. [J]. Journal of Chongqing Medical University (2):162-165(2003)
[1]曹前,吴家廉,骆文龙.反义寡核苷酸抑制端粒酶活性对鼻咽癌细胞癌基因表达的影响[J].重庆医科大学学报,2003(02):162-165. DOI： 10.13406/j.cnki.cyxb.2003.02.013.

CAO Qian, et al. The influence of antisense oligodeoxynucleotide targeting against telomerase in the expression of cellular oncogene to nasopharyngeal carcinoma cells. [J]. Journal of Chongqing Medical University (2):162-165(2003) DOI： 10.13406/j.cnki.cyxb.2003.02.013.

摘要

目的 :检测针对端粒酶的反义寡核苷酸（AS -ODN）对鼻咽癌HNE - 1细胞株bcl- 2及c -myc基因表达的影响。方法 :用PCR -ELISA法检测细胞内端粒酶活性。用免疫细胞化学法检测bcl- 2和c -myc基因的表达。结果 :鼻咽癌细胞株HNE - 1与 2 0 μmol/L反义寡核苷酸共同孵育 1天

对其端粒酶活性抑制率为 6 1.6 %。而同浓度正义寡核苷酸（S -ODN）对细胞端粒酶活性无抑制作用。 2 0 μmol/L反义寡核苷酸作用 2天后鼻咽癌细胞c -myc基因表达HSCORE评分由 3.6 5下降为 1.98

而bcl- 2基因表达HSCORE评分无明显变化。结论 :该反义寡核苷酸可抑制鼻咽癌细胞端粒酶活性

此作用有序列特异性。该反义寡核苷酸抑制了鼻咽癌细胞c-myc基因的表达

而对bcl- 2基因表达无明显影响。

Abstract

Objective: To investigate the influence of AS-ODN targeting against telomerase in the expression of bcl-2 gene and c-myc gene to nasopharyngeal carcinoma cells. Methods: Telomerase activity of HNE-1 cell strain was analysed by PCR-ELISA technique.The expressions of bcl-2 gene and c-myc gene were detected by immunocytochemical method. Results: HNE-1 cells were incubated with 20μ mol/L AS-ODN for 1 day

the inhibition rate of telomerase activity was 61.6% . The S-ODN did not inhibit telomerase activity of HNE-1 cell strain at the same concentration . The score of HSCORE to c-myc gene was turned down from 3.65 to l.98 when HNE-1 cells were incubated with 20μmol/L AS-ODN for 2 days

while it did not change significantly to bcl-2 gene at the same time. Conclusion: The AS-ODN inhibits telomerase activity of HNE-1 cell strain.This procedure is sequential specific.The AS-ODN inhibits the expression of c-myc gene

but has no significant influence in the expression of bcl-2 gene.

关键词

反义寡核苷酸端粒酶癌基因

Keywords

Antisense oligodeoxynucleotidetelomeraseOncogene

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Related Institution

重庆医科大学第二临床学院耳鼻咽喉科

贵州安顺市第二人民医院五官科

Deparment of Otorhinolaryngology, the Second College of Clinical Medicine, Chongqing Medical University

重庆医科大学检验系临床生化室主治医师

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