Intracellular free calcium and calmodulin expression in a brain edema model induced by endotoxin in infant rats

LI Mei; et al

doi:10.13406/j.cnki.cyxb.2003.01.016

您当前的位置：

首页 >

文章列表页 >

Intracellular free calcium and calmodulin expression in a brain edema model induced by endotoxin in infant rats

更新时间：2024-01-17

- Intracellular free calcium and calmodulin expression in a brain edema model induced by endotoxin in infant rats
- Journal of Chongqing Medical University Issue 1, Pages: 53-56(2003)
- 作者机构：
  
  1. 重庆医科大学儿科学院神经内科
  2. 重庆医科大学儿科学院神经内科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.01.016
  CLC： R741
- Published：2003，
扫描看全文
[1]李梅,蔡方成.幼年鼠内毒素性脑水肿模型及脑组织钙离子和钙调素表达的研究[J].重庆医科大学学报,2003(01):53-56.

LI Mei, et al. Intracellular free calcium and calmodulin expression in a brain edema model induced by endotoxin in infant rats. [J]. Journal of Chongqing Medical University (1):53-56(2003)
[1]李梅,蔡方成.幼年鼠内毒素性脑水肿模型及脑组织钙离子和钙调素表达的研究[J].重庆医科大学学报,2003(01):53-56. DOI： 10.13406/j.cnki.cyxb.2003.01.016.

LI Mei, et al. Intracellular free calcium and calmodulin expression in a brain edema model induced by endotoxin in infant rats. [J]. Journal of Chongqing Medical University (1):53-56(2003) DOI： 10.13406/j.cnki.cyxb.2003.01.016.

摘要

目的:建立简单、易复制的幼年鼠内毒素性脑水肿模型

并从Ca

、钙调素（CaM）水平探讨脑水肿的发生发展。方法:45只幼鼠

随机分内毒素组（36只）和对照组（9只）

分别于腹腔内注射内毒素（LPS）10mg/kg和等容量生理盐水

并用电镜观察其病理、用常规生化法测定脑含水量和伊文氏蓝（EB）含量、用荧光标记术和免疫印迹法分别测定脑组织细胞内[Ca

]和CaM表达。结果:脑组织含水量和EB含量显著高于对照组;细胞内[Ca

]明显增高

脑组织CaM表达增强;电镜显示血脑屏障（Blood-brain barrier

BBB）受损、神经元变性、胶质细胞肿胀、坏死等特征。结论:LPS导致了BBB通透性改变

并引起了混合性脑水肿。LPS引起了细胞内[Ca

]增高

并激活了CaM

从而启动了Ca

-CaM信号通路

这可能与其增加BBB通透性并导致脑水肿形成有密切关系。

Abstract

Objective :To establish a simple

reproducible brain edema model induced by endotoxin in infant rats and explore the role of intracellular free calcium and calmodulin (CaM). in the development of brain edema. Methods: A total of 45 infant rats were divided randomly into endotoxin treated( n = 36) and control( n = 9) group. The animals were intraperitoneally injected with 10mg/kg of endotoxin or saline respectively. The brain tissue intracelluar free [Ca2 + ] as well as the expression of CaM was detected by fluorescence and protein hybridizaton methods respectively. The tissue water content

Evans Blue content and pathology under electronic microscope were also observed. Results:Brain water and EB content were remarkably increased in endotoxin treated animals compared with those in the control

in which the intracellular free [Ca2+ ] was also significantly increased and the expression of CaM up - regulated. Electron microscopic studies revealed that the blood - brain barrier(BBB)was disrupted with neuronal degeneration

neurogliocyte swelling and necrosis. Conculsion :These results suggest that LPS increases the intracellular free [Ca2+ ]

and activates the CaM - dependent function via Ca2+ /CaM pathway

which might relate to the increased BBB permeability and brain edema formation.

关键词

内毒素脑水肿钙离子钙调素

Keywords

endotoxinbrain edemaCalciumCalmodulin

references

Views

126

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

THE EFFECT OF CHRONIC LITHIUM. ON CALMODULIN ACTIVITY IN RAT BRAIN

STUDIES ON THE PROTECTIVE EFFECTS OF PAF RECEPTOR ANTAGO-NISTS ON THE RED BLOOD CELL MEMBRANE INJURY INDUCED BY ENDOTOXIN ADMINISTRATION IN WISTAR RATS

Effects of gargle of composite of phenols in tea on subgingival bacteria and levels of endotoxin in gingival crevicular fluid of periodontitis

The mechanism of brain edema induced by tumor necrosis factor-alpha

Establishment of models of chronic pancreatitis with ethanol and endotoxin

Related Author

周保和

Yang Yi et al Department of physiology

et al

Lu Liying

唐明

张伟

罗慧琼

et al

Related Institution

重庆医科大学生理教研室

Dept.of Clincial Biochemistry

Department of Stomatology,the First Affiliated Hospital,Chongqing Medical University

重庆市药品检验所

Department of Neurosurgery,the First Affiliatied Hospital,Chongqing Medical University

⁰