The study of bladder cancer cell line irradiated with high intensity focused ultrasound

LIU Chuan; et al

doi:10.13406/j.cnki.cyxb.2003.01.010

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The study of bladder cancer cell line irradiated with high intensity focused ultrasound

更新时间：2024-01-17

- The study of bladder cancer cell line irradiated with high intensity focused ultrasound
- Journal of Chongqing Medical University Issue 1, Pages: 32-35(2003)
- 作者机构：
  
  1. 重庆医科大学第二临床学院泌尿外科
  2. 重庆医科大学医学超声工程研究所,重庆,400010
  3. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.01.010
  CLC： R737.14
- Published：2003，
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[1]刘川,乔天愚,杨光永,张唯力,伍烽,王智彪.高强度聚焦超声破坏膀胱癌细胞株的实验研究[J].重庆医科大学学报,2003(01):32-35.

LIU Chuan, et al. The study of bladder cancer cell line irradiated with high intensity focused ultrasound. [J]. Journal of Chongqing Medical University (1):32-35(2003)
[1]刘川,乔天愚,杨光永,张唯力,伍烽,王智彪.高强度聚焦超声破坏膀胱癌细胞株的实验研究[J].重庆医科大学学报,2003(01):32-35. DOI： 10.13406/j.cnki.cyxb.2003.01.010.

LIU Chuan, et al. The study of bladder cancer cell line irradiated with high intensity focused ultrasound. [J]. Journal of Chongqing Medical University (1):32-35(2003) DOI： 10.13406/j.cnki.cyxb.2003.01.010.

摘要

目的:探讨高强度聚焦超声（HIFU）治疗膀胱癌的可行性。方法:HIFU辐照T

膀胱癌细胞株后用MTT法测定活细胞数

分析HIFU剂量与细胞存活率的关系

测定培养液内碱性磷酸酶（ALP）、肌酸激酶（CK）活性

并对癌细胞进行形态学观察。结果:随着HIFU剂量增加

膀胱癌细胞存活率迅速减少

残留癌细胞生长受抑。癌细胞结构受到不可逆性破坏。结论:HIFU治疗膀胱癌是可行的。

Abstract

Objective:To investigate the feasibility and mechanism of high intensity focused ultrasound (HIFU) in the treatment of bladder cancer. Methods:After irradiating the bladder cancer cell line T24

the number of survival cells was detected with MTT col-orimetry. The relationship between HIFU dosage and cell survival rate was analysed. The activity of alkaline phosphatase (ALP) and creatine- kinase (CK) in culture medium was determined and the structures of the cells were studied. Results: The relationship between sound intensity and cell survival rate is in power series. With increasing sound intensity

cell survival rate decreased sharply. The relationship between irradiation time and cell survival rate is linear. When sound intensity was 2300 W/cm2

the optimum irradiation time was 7 seconds. After irradiation

cell growth was suppressed and cell numbers reduced continuously until 6 hours later. This indicated that there was necrobiosis in these cells. With the prolongation of the irradiation

the activity of ALP、CK in culture medium increased and reached it’s peak at two seconds. Under optical and electronic microscopes

the damage to the cancer cell was irreversible. Conclusion :HIFU is an effective

minimally invasive treatment option for bladder tumor.

关键词

高强度聚焦超声膀胱肿瘤细胞株超声治疗

Keywords

high intensity focused ultrasoundBladder tumorCell lineultrasound therapy

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重庆医科大学临床学院泌尿外科

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