Rapid amplification of the ends of cDNA fragment from an unknown KL gene

LIU Beizhong; et ai

doi:10.13406/j.cnki.cyxb.2003.01.005

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Rapid amplification of the ends of cDNA fragment from an unknown KL gene

更新时间：2024-01-17

- Rapid amplification of the ends of cDNA fragment from an unknown KL gene
- Journal of Chongqing Medical University Issue 1, Pages: 14-16(2003)
- 作者机构：
  
  1. 重庆医科大学临床生化教研室
  2. 重庆医科大学临床学院血液科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.01.005
  CLC： R73-3
- Published：2003，
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[1]刘北忠,蒋纪恺,何於娟,张彦,刘小珊.未知KL基因cDNA片段的末端快速扩增[J].重庆医科大学学报,2003(01):14-16+28.

LIU Beizhong, et ai. Rapid amplification of the ends of cDNA fragment from an unknown KL gene. [J]. Journal of Chongqing Medical University (1):14-16(2003)
[1]刘北忠,蒋纪恺,何於娟,张彦,刘小珊.未知KL基因cDNA片段的末端快速扩增[J].重庆医科大学学报,2003(01):14-16+28. DOI： 10.13406/j.cnki.cyxb.2003.01.005.

LIU Beizhong, et ai. Rapid amplification of the ends of cDNA fragment from an unknown KL gene. [J]. Journal of Chongqing Medical University (1):14-16(2003) DOI： 10.13406/j.cnki.cyxb.2003.01.005.

摘要

目的:为研究白血病K562细胞中未知KL基因的结构与功能

对其cDNA片段进行全长克隆。方法:采用3种RACE策略

对未知KL基因的cDNA片段进行末端的快速扩增。结果:网上LabOnWeb的instant RACE与传统的3′-RACE使未知KL基因的cDNA片段向5′末端与3′末端各自延伸了156bp与350bp。结论:通过末端快速扩增

表明白血病K562细胞中未知KL基因的全长cDNA大小约为1Kb

这为我们进行该基因的全长克隆

进而研究其结构与功能奠定了基础。

Abstract

Objective:To do the full - length cloning of the cDNA fragment of an unknown KL gene in leukemia K562 cell for study of its structure and function. Methods : The ends of the cDNA fragment of the unknown KL gene were amplified using three strategies of RACE. Results: 5’ - end of the cDNA fragment of the unknown KL gene was extended 156 base pairs by Labon Web instance RACE online and 3’ - end was extended 350 base pairs by traditional 3’ - RACE. Conclusion : It suggests that the full length of the cDNA of the unknown KL gene in leukemia K562 cell is approximately composed of one kilo- base by RACE. It is helpful to full -length cloning of the unknown gene

also to its structure and function study.

关键词

苦参碱K562细胞RACE新基因

Keywords

MatrineK562 cellRACENew gene

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Related Institution

Department of Clinical Biochemistry, Chongqing Medical University

重庆医科大学检验系临床生化教研室

Department of Clinical Laboratory Medicine,Chongqing Medical University

Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University

重庆医科大学附属第二医院消化内科

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