The effects of ascorbic acid on activity of PKC - in rats with streptozotocin - induced diabetes

XIAO Qian; et al

doi:10.13406/j.cnki.cyxb.2003.01.003

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The effects of ascorbic acid on activity of PKC - in rats with streptozotocin - induced diabetes

更新时间：2024-01-17

- The effects of ascorbic acid on activity of PKC - in rats with streptozotocin - induced diabetes
- Journal of Chongqing Medical University Issue 1, Pages: 8-10(2003)
- 作者机构：
  
  1. 重庆医科大学临床学院内分泌科
  2. 重庆医科大学临床学院内分泌科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.01.003
  CLC： R587.1
- Published：2003，
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[1]肖谦,汪恕萍.抗坏血酸对糖尿病大鼠肾脏PKC-β_1活化的影响[J].重庆医科大学学报,2003(01):8-10+13.

XIAO Qian, et al. The effects of ascorbic acid on activity of PKC - in rats with streptozotocin - induced diabetes. [J]. Journal of Chongqing Medical University (1):8-10(2003)
[1]肖谦,汪恕萍.抗坏血酸对糖尿病大鼠肾脏PKC-β_1活化的影响[J].重庆医科大学学报,2003(01):8-10+13. DOI： 10.13406/j.cnki.cyxb.2003.01.003.

XIAO Qian, et al. The effects of ascorbic acid on activity of PKC - in rats with streptozotocin - induced diabetes. [J]. Journal of Chongqing Medical University (1):8-10(2003) DOI： 10.13406/j.cnki.cyxb.2003.01.003.

摘要

目的:探讨抗坏血酸对糖尿病大鼠肾脏蛋白激酶C-β

（PKC-β

）活化的影响。方法:Wistar大鼠随机分为3组

正常对照组、糖尿病组和糖尿病治疗组。实验第9周测定血浆和肾脏抗坏血酸水平

免疫荧光检测和激光共聚焦显微镜观察PKC-β

活化水平。结果:与正常对照组相比

糖尿病组血浆、肾脏抗坏血酸水平显著降低（P

0.05）;肾脏PKC-β

活化水平显著增加（P

0.05）;糖尿病治疗组8周后血浆、肾脏抗坏血酸水平明显增高

肾脏PKC-β

活化水平显著下降

与正常对照组相比差异尤显著性（P

0.05）。结论:糖尿病时肾脏PKC-β

活化增加可能与体内抗坏血酸水平降低有关

补充抗坏血酸可抑制糖尿病大鼠肾脏PKC-β

活化。

Abstract

Objective :To study the effects of ascorbic acid(vitamin C) on PKC -β1 activity in kidneys of diabetic rats. Methods :30 Wistar rats were randomly divided into three groups: Control group (group C)

streptozotocin - induced diabetic rats group(group 13) and diabetic rats treated with ascorbic acid 90mg/(kg·d)(group DT). The concentration of ascorbic acid in plasma and kidney were measured at 9 week. The activity of PKC - β1 was observed by immunofluorescence technique and laser confocal microscope. Results : Compared with group C

the level of ascorbic acid in the plasma and kidney of group D decreased significantly (P<0.05)

and the activity of PKC - β1 was markedly increased. After 8 weeks of treatment with ascorbic acid

the PKC - β1 activity was nearly normalized and the level of ascorbic acid in the plasma and kidney was increased significantly. Conclusion : This finding suggests that the increased PKC - ft activity in kidneys of diabetic rats may be associated with lower levels of ascorbic acid. Administration of ascorbic acid can inhibit the PKC- β1 activation in kidneys of diabetic rats.

关键词

抗坏血酸糖尿病大鼠蛋白激酶C-β1

Keywords

Ascorbic acidDiabetic ratsProtein kinase C-β1

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重庆医科大学临床学院眼科

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