The isolation and culture of rat hepatic stellate cells with collagenase in situ liver recirculating perfusion

LUO Yun; et al

doi:10.13406/j.cnki.cyxb.2002.01.015

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The isolation and culture of rat hepatic stellate cells with collagenase in situ liver recirculating perfusion

更新时间：2024-01-17

- The isolation and culture of rat hepatic stellate cells with collagenase in situ liver recirculating perfusion
- Journal of Chongqing Medical University Issue 1, Pages: 48-49(2002)
- 作者机构：
  
  1. 重庆医科大学第二临床学院消化内科
  2. 重庆医科大学病毒性肝炎研究所,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2002.01.015
  CLC： R575.2
- Published：2002，
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[1]罗云,戴立里,沈鼎明,姚云清,张大志,王波.原位循环灌流法分离大鼠肝星状细胞[J].重庆医科大学学报,2002(01):48-49+52.

LUO Yun, et al. The isolation and culture of rat hepatic stellate cells with collagenase in situ liver recirculating perfusion. [J]. Journal of Chongqing Medical University (1):48-49(2002)
[1]罗云,戴立里,沈鼎明,姚云清,张大志,王波.原位循环灌流法分离大鼠肝星状细胞[J].重庆医科大学学报,2002(01):48-49+52. DOI： 10.13406/j.cnki.cyxb.2002.01.015.

LUO Yun, et al. The isolation and culture of rat hepatic stellate cells with collagenase in situ liver recirculating perfusion. [J]. Journal of Chongqing Medical University (1):48-49(2002) DOI： 10.13406/j.cnki.cyxb.2002.01.015.

摘要

目的：为深入探讨肝纤维化的细胞机制，探索经济、稳定的肝星状细胞分离和培养方法。方法：采用胶原酶原位循环灌流法分离大鼠肝星状细胞，１８％Ｎｙｃｏｄｅｎｚ密度梯度离心纯化星状细胞。结果：星状细胞的得率为１×１０７～３×１０７／肝，纯度为９０％，存活率为９６％。结论：原位循环灌流法是有效的大鼠肝星状细胞的分离和培养方法。

Abstract

Objective: To investigate the method of isolation and culture of hepatic stellate cells for studying the cellular mechanisms of hepatic fibrosis. Methods: The isolation of normal rat hepatic stellate cells was established with collagenase in situ liver recirculating perfusion.Hepatic stellate cells were purified by using density gradient centrifugation with 18% Nycodenz. Results:The amount of hepatic stellate cells obtained were 1×107～3×107 cells per liver with a purity of more than 90% and the viability of more than 96%. Conclusion: The result showed that the methods of the isolation and culture of rat hepatic setllate cells were economical and steady.

关键词

肝星状细胞细胞分离原位循环灌流

Keywords

hepatic stellate cellcell isolationIn situ liver recirculating perfusion

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