Screening the optimized condition of RAPD - POP

PU Shu - ping; et al

doi:10.13406/j.cnki.cyxb.2001.04.025

您当前的位置：

首页 >

文章列表页 >

Screening the optimized condition of RAPD - POP

更新时间：2024-01-17

- Screening the optimized condition of RAPD - POP
- Journal of Chongqing Medical University Issue 4, Pages: 436-438(2001)
- 作者机构：
  
  1. 重庆医科大学基础医学院生物学教研室
  2. 重庆职工医学院生物学教研室
  3. 重庆医科大学基础医学院生物学教研室,重庆,400016
  4. ,重庆,400050
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2001.04.025
  CLC： R346
- Published：2001，
扫描看全文
[1]蒲淑萍,王希,段昌柱.RAPD的建立及优化研究[J].重庆医科大学学报,2001(04):436-438+442.

PU Shu - ping, et al. Screening the optimized condition of RAPD - POP. [J]. Journal of Chongqing Medical University (4):436-438(2001)
[1]蒲淑萍,王希,段昌柱.RAPD的建立及优化研究[J].重庆医科大学学报,2001(04):436-438+442. DOI： 10.13406/j.cnki.cyxb.2001.04.025.

PU Shu - ping, et al. Screening the optimized condition of RAPD - POP. [J]. Journal of Chongqing Medical University (4):436-438(2001) DOI： 10.13406/j.cnki.cyxb.2001.04.025.

摘要

目的：建立优化的ＲＡＰＤ反应条件，为进一步迸行遗传监控研究奠定基础。方法：按常规方法制备小鼠基因组ＤＮＡ，在一定的ＲＡＰＤ－ＰＣＲ反应条件下分别改变各组份浓度及退火温度。扩增产物在含溴化乙锭的１．５％琼脂糖凝胶中电泳，紫外透射仪上观察照相。结果：当Ｍｇ２＋浓度为１．５ｍｍｏｌ／Ｌ，４种ｄＮＴＰｓ各为１５０ｕｍｏｌ／Ｌａｑ酶为１５Ｕ，引物为０．２ｍｏｌ／Ｌ，模板ＤＮＡ为５０ｎｇ，退火温度为３８℃，ＲＡＰＤ－ＰＣＲ扩增效果好。结论：在优化的条件下规范操作，ＲＡＰＤ的稳定性、重复性好。

Abstract

Objective To establish the optimized RAPD - PCR reaction conditions for further genetic monitoring study. Methods Altering the concentrations of the reaction components and the annealing temperature of RAPD - PCR

PCR products from RAPD were electrophoretically separated in agarose gels and banding profiles were visualized by ethidium bromide staining. Recults RAPD - PCR reactions were performed in duplication with SOng of genomic DNA

lSOpmol/L each dNTP

0. 2xmol/L primer

1.5mmol/L magnesium chloride

I.. 5 units of Taq polymerase

annealing at 38t . Under these conditions RAPD method is stable and reproducible. Conclusion The optimized RAPD - PCR reaction conditions were established in this study.

关键词

RAPD基因组DNA优化反应条件

Keywords

Random amplified polymorphic DNA( RAPD)genomic DNAOptimal reaction conditions

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Protocol and application of ethanol method to extract genomic DNA from transgenic mouse embryos

Related Author

WENG Minjie

DU Jianlin

PU Di

ZHANG Jin

LI Xiaoqun

LIU Yajie

SHE Qiang

Related Institution

(Department of Cardiology,the Second Affiliated Hospital,Chongqing Medical University)

⁰