Effect of ischemic preconditioning plus cardioplegic solutions on immature rabbit heart function and morphology

ZHU Bin; et al

doi:10.13406/j.cnki.cyxb.2001.04.015

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Effect of ischemic preconditioning plus cardioplegic solutions on immature rabbit heart function and morphology

更新时间：2024-01-17

- Effect of ischemic preconditioning plus cardioplegic solutions on immature rabbit heart function and morphology
- Journal of Chongqing Medical University Issue 4, Pages: 407-409(2001)
- 作者机构：
  
  1. 重庆医科大学临床学院麻醉科
  2. 中国医学科学院阜外医院麻醉体外循环研究室,重庆,400016
  3. ,北京,100037
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2001.04.015
  CLC： R614
- Published：2001，
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[1]朱斌,闵苏,龙村.缺血预处理联合St.ThomasⅡ心麻痹液对兔未成熟心脏功能和病理学影响[J].重庆医科大学学报,2001(04):407-409+415.

ZHU Bin, et al. Effect of ischemic preconditioning plus cardioplegic solutions on immature rabbit heart function and morphology. [J]. Journal of Chongqing Medical University (4):407-409(2001)
[1]朱斌,闵苏,龙村.缺血预处理联合St.ThomasⅡ心麻痹液对兔未成熟心脏功能和病理学影响[J].重庆医科大学学报,2001(04):407-409+415. DOI： 10.13406/j.cnki.cyxb.2001.04.015.

ZHU Bin, et al. Effect of ischemic preconditioning plus cardioplegic solutions on immature rabbit heart function and morphology. [J]. Journal of Chongqing Medical University (4):407-409(2001) DOI： 10.13406/j.cnki.cyxb.2001.04.015.

摘要

目的：研究缺血预处理（ＩＰＣ）对兔未成熟心肌缺血再灌注损伤的影响，并探讨其可能机制。方法：利用Ｌａｎｇｅｎｄｏｒｆｆ模型灌注幼兔（１４—２１天）离体心脏。１８只幼兔心随机分为２组，每组９只ＩＰＣ组经历５ｍｉｎ缺血、１０ｍｉｎ再灌的ＩＰＣ处理后，使用Ｓｔ．ＴｈｏｍａｓⅡ心麻痹液（ＳＴＨ）使心脏停跳；对照组只用ＳＴＨ停跳心脏。两组兔心均在生理体温（３９℃）下接受４５ｍｉｎ缺血、４０ｍｉｎ灌注。观察复灌后的心肌酶释放、心肌能量、心脏功能及病理学变化。结果：与对照组相比，ＩＰＣ组肌酸磷酸激酶同工酶（ＣＫ－ＭＢ）漏出量明显减少（Ｐ＜０．０１），心肌ＡＴＰ含量以保存（Ｐ＜０．０５），再灌注末心脏左室发展压（ＬＶＤＰ）显著改善（Ｐ＜０．０５）；光、电镜显示经过缺血预处理的心肌细胞损伤轻。结论：缺血预处理能明显减轻兔未成熟心肌缺血再灌注损伤，改善再灌注后心脏功能的恢复，其机制可能与保存再灌注后心肌细胞中ＡＴＰ含量有关。

Abstract

Objective: To investigate whether immature hearts experiencing global ischemia and reperfusion injury could be protected by ischemic preconditioning( IPC) and probe the possible mechanism. Methods : Aerobically perfused in Langendorff mode at 39t

isolated immature rabbit hearts( 14 -21 days old)were subjected to a IPC stimulus consisting of 5 minutes of global ischenmia plus 10 minutes of reperfusion. This was followed by 45 minutes of global ischemia and 40 minutes of reperfusion. Left ventricular developed pressure( LVDP)

change of myocardial enzyme (CK - MB) in the coronary effluent

myocardial energy metabolism and morphology were de termined. Cold crystalloid cardioplegia was used to cause the hearts to arrest before the prolonged global I/R. Re sults : The LVDP value in IPC group was significantly higher than that in control. The CK - MB leakage in IPC group was decreased significantly (24.58 ± 5.09 U/L versus 32.80 ± 5.32 U/L in controls P<0. 01). The myocardial levels of ATP in preconditioned group at the end of the reperfusion was significantly preserved(208. 14 ±24. 2ug /g versus 174.52 ± 15. 69ugg/g in controls P<0. 05). Light and electron microscopic findings of myocardium showed the preconditioned hearts were less severely damaged. Conclusion : IPC has a protective effect on the immature rabbit hearts suffering from global I /R injury

which is possibly connected with the preservation of ATP level in myocardium tissue.

关键词

缺血预处理未成熟心麻痹液

Keywords

ischemic preconditioningImmatureCardioplegic solutions

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