Quantification of bone alkaline phosphatase in serum by wheat-germ lectin precipitation

WANG Hua; et al

doi:10.13406/j.cnki.cyxb.2001.04.013

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Quantification of bone alkaline phosphatase in serum by wheat-germ lectin precipitation

更新时间：2024-01-17

- Quantification of bone alkaline phosphatase in serum by wheat-germ lectin precipitation
- Journal of Chongqing Medical University Issue 4, Pages: 402-404(2001)
- 作者机构：
  
  1. 重庆医科大学儿科学院儿保科
  2. 重庆医科大学儿科学院儿科研究所
  3. 重庆医科大学儿科学院检验科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2001.04.013
  CLC： R446.1
- Published：2001，
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[1]王华,黎海芪,杨锡强,李继斌,瞿平,刘瑜,胡怡.麦芽凝集素沉淀法检测骨碱性磷酸酶同工酶及应用[J].重庆医科大学学报,2001(04):402-404.

WANG Hua, et al. Quantification of bone alkaline phosphatase in serum by wheat-germ lectin precipitation. [J]. Journal of Chongqing Medical University (4):402-404(2001)
[1]王华,黎海芪,杨锡强,李继斌,瞿平,刘瑜,胡怡.麦芽凝集素沉淀法检测骨碱性磷酸酶同工酶及应用[J].重庆医科大学学报,2001(04):402-404. DOI： 10.13406/j.cnki.cyxb.2001.04.013.

WANG Hua, et al. Quantification of bone alkaline phosphatase in serum by wheat-germ lectin precipitation. [J]. Journal of Chongqing Medical University (4):402-404(2001) DOI： 10.13406/j.cnki.cyxb.2001.04.013.

摘要

目的：应用麦芽凝集素（ＷＧＡ）沉淀法检测骨碱性磷酸酶同工酶（Ｂ－ＡＬＰ）水平，并探索Ｂ—ＡＬＰ与骨矿含量的相关性。方法：建立维生素Ｄ和钙缺乏所致的佝偻病动物模型，测定骨矿含量和血清钙、磷、２５－ＯＨＤ３、总碱性磷酸酶（Ｔ—ＡＬＰ）水平；采用ＷＧＡ沉淀法检测不同生长阶段大鼠血清中的Ｂ—ＡＬＰ活性。结果：血清Ｂ—ＡＬＰ在佝偻病组明显升高，且出现时间较Ｔ—ＡＬＰ早；Ｂ—ＡＬＰ与同期骨矿含量呈显著负相关。结论：血清Ｂ—ＡＬＰ活性是反映成骨细胞功能更为敏感和可靠的指标。

Abstract

Objective: To determine the serum levels of bone alkaline phosphatase (B - ALP) by means of wheat - germ Iectin precipitation and explore correlation between B - ALP and bone mineral content. Methods: The animal model with calcium and vitamin D deficient rickets were established. Serum levels of calcium

phos phorus

25 - OHD3

total ALP and bone ALP in different growth phase were assayed. Results : Bone ALP activity was significantly increased in rickets group and this increase occurred earlier than that of the total ALP. There was a significant negative correlation between bone ALP and bone mineral content. Conclusion : Serum bone ALP activity is a sensitive and reliable index in measuring osteoblastic activity.

关键词

碱性磷酸酶同工酶骨矿含量佝偻病

Keywords

alkaline phosphataseIsoenzymesBone mineral contentRickets

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