The observation of androgen receptor and estrogen receptor in hepatocellular carcinomas with immunoelectron - microscopy

LUO Fang.etal

doi:10.13406/j.cnki.cyxb.2001.03.013

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The observation of androgen receptor and estrogen receptor in hepatocellular carcinomas with immunoelectron - microscopy

更新时间：2024-01-17

- The observation of androgen receptor and estrogen receptor in hepatocellular carcinomas with immunoelectron - microscopy
- Journal of Chongqing Medical University Issue 3, Pages: 264-267(2001)
- 作者机构：
  
  1. 重庆医科大学临床学院普外科
  2. 重庆医科大学电镜室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2001.03.013
  CLC： R735.7
- Published：2001，
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[1]罗放,马绍华,唐时森,陆有生,徐晨,王红.雄激素受体、雌激素受体在肝细胞型肝癌中的免疫电镜观察[J].重庆医科大学学报,2001(03):264-267.

LUO Fang.etal. The observation of androgen receptor and estrogen receptor in hepatocellular carcinomas with immunoelectron - microscopy. [J]. Journal of Chongqing Medical University (3):264-267(2001)
[1]罗放,马绍华,唐时森,陆有生,徐晨,王红.雄激素受体、雌激素受体在肝细胞型肝癌中的免疫电镜观察[J].重庆医科大学学报,2001(03):264-267. DOI： 10.13406/j.cnki.cyxb.2001.03.013.

LUO Fang.etal. The observation of androgen receptor and estrogen receptor in hepatocellular carcinomas with immunoelectron - microscopy. [J]. Journal of Chongqing Medical University (3):264-267(2001) DOI： 10.13406/j.cnki.cyxb.2001.03.013.

摘要

目的：观察雄激素受体（ＡＲ）与雌激素受体（ＥＲ）在肝癌和非癌肝组织超微结构上的分布。探讨ＡＲ、ＥＲ在肝癌致癌过程中的作用。方法：用免疫胶体金染色法（ＩＧＳ）标记１３例肝细胞型肝癌与５例非癌肝组织细胞内雄激素受体（ＡＲ）及雌激素受体（ＥＲ），并用免疫透射电镜观察。结果：发现胶体金颗粒主要分布于线粒体双层膜和脊膜；粗面内质网；结合及游离核蛋白体；核膜及核周间隙，异染色质及常染色质等部位。统计分析表明，肝癌细胞金颗粒明显多于非癌肝细胞内金颗粒（Ｐ＜０．０５）。肝癌细胞胞质、胞核ＡＲ金颗粒明显多于ＥＲ金颗粒（Ｐ＜０．０５）。而非癌肝细胞胞质、胞核ＡＲ、ＥＲｋ颗粒数量无显著差别（Ｐ＞０．０５）。结论：提示肝癌是一种雄激素依赖肿瘤，为抗性激素治疗肝癌提供了理论依据。

Abstract

Objective :To observe distribution on the ultrastructure of androgen receptor (AR) and estrogen receptor (ER) in hepatocellular carcinomea (Hcc) and non - cancerous liver. To make clear the effect of AR and ER in the course that cause cancer in Hcc. Methods: Using the method of immunogold staining androgen receptor (AR) and estrogen receptor (ER) in 13 cases of hepatocellular carcinomas (Hcc) and 5 cases of noncancerous liver and observing with transmission electron microscope. Results-.Gold granules were mainly distributed over the double - layered membranes of mitochondria

memebranes of rough endoplasmic reticulum and some smooth endoplasmic reticulum

bound and free ribosomes

nuclear membranes and perinuclear cristerna and heterochro- matin and euchromatin

etc. The statistics indicate that the number of gold granules in cancer cells is greater than that in non- cancerous liver cells (P<0.05). In plasma and nucleus of cancer cells

the gold granules of AR is more than those of ER( P < 0.05 )

while no difference exists between plasma and mucleus of non- cancerous liv- er (P >0.05). Conclusion-. It indicates that Hcc is a kind of androgen - depenedent tumor

providing the theo- retical basis for anti - androgen therapy.

关键词

肝细胞型肝癌雄激素受体雌激素受体免疫金染色

Keywords

Hepatocellular carcinomaandrogen receptorestrogen receptorImmunogold staining

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