The study of human leukemia cell K562 transfected with B7 gene

JIANG Dai-feng; et al

doi:10.13406/j.cnki.cyxb.2001.03.002

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The study of human leukemia cell K562 transfected with B7 gene

更新时间：2024-01-17

- The study of human leukemia cell K562 transfected with B7 gene
- Journal of Chongqing Medical University Issue 3, Pages: 228-230(2001)
- 作者机构：
  
  1. 重庆医科大学临床血液教研室
  2. 重庆医科大学肝炎研究所,重庆,400046
  3. .,重庆,400046
  4. ,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2001.03.002
  CLC： R733.7
- Published：2001，
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[1]蒋代凤,罗云萍,任红,凌宁,陈洁萍.B7转染白血病细胞K562的作用研究[J].重庆医科大学学报,2001(03):228-230+240.

JIANG Dai-feng, et al. The study of human leukemia cell K562 transfected with B7 gene. [J]. Journal of Chongqing Medical University (3):228-230(2001)
[1]蒋代凤,罗云萍,任红,凌宁,陈洁萍.B7转染白血病细胞K562的作用研究[J].重庆医科大学学报,2001(03):228-230+240. DOI： 10.13406/j.cnki.cyxb.2001.03.002.

JIANG Dai-feng, et al. The study of human leukemia cell K562 transfected with B7 gene. [J]. Journal of Chongqing Medical University (3):228-230(2001) DOI： 10.13406/j.cnki.cyxb.2001.03.002.

摘要

目的：探讨共刺激信号分子Ｂ７转化人白血病细胞株Ｋ５６２后，转化细胞的生物学性状变化及介导的免疫作用。方法：构建重组真核表达质粒ｐｃＤＮＡ３．１Ｂ７，转化人白血病细胞株Ｋ５６２，获得单个细胞克隆，观察Ｋ５６２－Ｂ７的生长周期，绘制生长曲线，检测Ｋ５６２－Ｂ７诱导Ｔ细胞分泌ＩＬ－２的情况。结果：ｐｃＤＮＡ３．１Ｂ７可在Ｋ５６２中稳定表达，Ｋ５６２－Ｂ７的生长增殖速度比野生型Ｋ５６２慢。在体外，Ｋ５６２－Ｂ７可诱导ＰＭＮＣ分泌ＩＬ－２，２４ｈ上清液中ＩＬ－２含量比Ｂ７阴性Ｋ５６２高１倍左右。结论：Ｂ７基因转入人白血病细胞株Ｋ５６２后，细胞本身增殖能力降低，且Ｋ５６２—Ｂ７可活化ＣＤ＋４Ｔ细胞分泌细胞因子ＩＬ－２，提高宿主抗肿瘤免疫能力。

Abstract

Objective： To assess the potential of costimul atory signal molecule B7 in inducing immune effect and the biological alternatio n of K562-B7.Methods： First

insert B7 gene into eukaryotic expressionplasmid pcDNA3.1B7

then transfect K562 with plasmid pcDNA3.1B7 and select positi ve clone(K562-B7) by G418(600μg/ml).Finaly

analyse the ability of growth and pr oliferation of K562-B7

The secretion of IL-2 was also observed in vitro.Re sults： pcDNA3.1B7 can be stably expressed in K562

and K562-B7 grow more slo wly than wild K562.In addition

IL-2 production per 24 hours in the supernatant stimulated by K562-B7 was about twice as much as that by B7 negative K562. Conclusio n： K562 transfected with B7 gene grow more slowly than K562 and K562-B7 can activate T cell to produce IL-2

and enhance anti-tumor immunity.

关键词

B7肿瘤免疫K562转染

Keywords

B7tumor immunityK562Transfect

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