The apoptosis of spinal cord and spinal ganglion in the mouse embryo

WANG Wei-wei

doi:10.13406/j.cnki.cyxb.2001.03.001

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The apoptosis of spinal cord and spinal ganglion in the mouse embryo

更新时间：2024-01-17

- The apoptosis of spinal cord and spinal ganglion in the mouse embryo
- Journal of Chongqing Medical University Issue 3, Pages: 225-227(2001)
- 作者机构：
  
  重庆医科大学基础医学院组织胚胎学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2001.03.001
  CLC： R321
- Published：2001，
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[1]汪维伟.小鼠胚胎脊髓及脊神经节中的细胞凋亡[J].重庆医科大学学报,2001(03):225-227.

WANG Wei-wei. The apoptosis of spinal cord and spinal ganglion in the mouse embryo. [J]. Journal of Chongqing Medical University (3):225-227(2001)
[1]汪维伟.小鼠胚胎脊髓及脊神经节中的细胞凋亡[J].重庆医科大学学报,2001(03):225-227. DOI： 10.13406/j.cnki.cyxb.2001.03.001.

WANG Wei-wei. The apoptosis of spinal cord and spinal ganglion in the mouse embryo. [J]. Journal of Chongqing Medical University (3):225-227(2001) DOI： 10.13406/j.cnki.cyxb.2001.03.001.

摘要

目的：观察小鼠胚胎脊髓（ｓｐｉｎａｌ　ｃｏｒｄ，ＳＣ）和脊神经节（ｓｐｉｎａｌ　ｇａｎｇｌｉｏｎ，ＳＧ）发生中的细胞凋亡。方法：以体视学方法观察Ｅ１２ｄ～Ｅ１５．５ｄ　ＩＣＲ胎鼠Ｈ．Ｅ染色石蜡连续切片中臂丛段、胸段、腰从段的ＳＣ和ＳＧ的凋亡细胞密度（Ｄｅｎｓｉｔｙ　ｏｆ　ａｐｏｐｔｏｔｉｃ　ｃｅｌｌ，ＤＡＣ）。部分切片用ＴＵＮＥＬ法标记凋亡细胞作对照。结果：胎鼠ＳＣ和ＳＧ中均见核碎裂的凋亡细胞。ＳＣ中的凋亡细胞主要分布在基板，偶见于翼板。统计结果显示，三段ＳＣ中的ＤＡＣ峰值均在Ｅ１４　ｄ，Ｐ＜０．０１；臂丛段的ＤＡＣ高于后两段，除Ｅ１５ｄ外，Ｐ均＜０．０５。胸段和腰从段ＳＧ中的ＤＡＣ峰值均在Ｅ１５ｄ，Ｐ＜０．０１；臂丛段的ＤＡＣ峰值不明显。结论：Ｅ１２ｄ～Ｅ１５．５ｄ胎鼠ＳＣ和ＳＧ中，凋亡细胞主要见于脊髓的基板和脊神经节。ＳＣ的ＤＡＣ峰值较ＳＧ早一天。ＤＡＣ的峰值可能与其组织分化有关。

Abstract

Objective：To observe the apoptosis of developi ng spinal cord and spinal ganglion in the mouse embryo. Methods：The density of apoptotic cell(DAC) of the brachial plexus

thoracic and lumbar plexus of spinal cord (SC)and spinal ganglion (SG)in the serial sections of fetal ICR mice aged 12～15.5 days was measured with the stereological method.The apo ptosis was also showed with TUNEL method in some sections for contrast.Re sults：The apoptotic cells with karyorrhexis can be seen in SC and SG of fetal mice.The apoptotic cells of SC are mainly located in the basal plate

but rarely in the alar plate.The statistical results showed that the peak values of DAC in the three sections of SC all appeared on day 14

P＜0.01; the peak value of DAC in the brachial plexus of SC was higher than the other two sections

P＜0.0 5

except day 15.The peak values of DAC in the thoracic and lumbar plexus of SG all appeared on day 15

P＜0.01;the DAC of brachial plexus was not clear.Conclusion ：The apoptotic cells in SC and SG of fetal mice aged 12～15.5 days were mainly found in the basal plate of SC and the SG.The pea k values of DAC in SC were one day earlier than in SG.The peak values of DAC in the three sections of SC and SG may be related with their tissue-differeniation.

关键词

细胞凋亡脊髓脊神经节小鼠胚胎体视学

Keywords

apoptosisSpinal cordSpinal ganglionFetal mous estereology

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