：Objective To study the effect of slow or quick freezing- storing on the function and ultrastructure of human spermatozoa with T-G type cryopreservative medium(CPM) as cryoprotectant during a longer period. Methods 45 normal semen samples were subjected to cryopreservation with or without T-G type CPM and slow or quick freezing- storing respectively. Fresh and frozen-thawed semen were examined by the routine analysis of semen and spermatozoa hypoosmotic swelling test and spermatozoa in vitro penetrate zona-free hamster ova assay and spermatozoa head ultrastructure observation and spermatozoa chromosome analysis. Results No spermatozoa in cryopreservated semen without T-G type of CPM was alive after thawed. The quality of frozen-thawed human semen by slow and quick freezing- storing methods with T-G type CPM was significantly lower than that of fresh semen(Ｐ<0.01) and the effect of slow freezing method was smaller than that of quick freezing method(Ｐ<0.01). Neither of the methods increased the rate of spermatozoa chromosome abnormalities and X/Y ratio of human spermatozoa(0.90<Ｐ<0.95). Conclusion Both cryopreservation methods have negative effect on human spermatozoa and the slow freezing method is better than the quick one. The sperm chromosome and X/Y spermatozoa ratio are not significantly affected by the two methods.