Study on the deletion and mutation of p16 gene in human non-small cell lung cancer and its clinical significance

doi:10.13406/j.cnki.cyxb.2000.04.013

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Study on the deletion and mutation of p16 gene in human non-small cell lung cancer and its clinical significance

更新时间：2024-01-17

- Study on the deletion and mutation of p16 gene in human non-small cell lung cancer and its clinical significance
- Journal of Chongqing Medical University Issue 4, Pages: 368-370(2000)
- 作者机构：
  
  1. 重庆医科大学临床学院胸心外科,重庆,400016
  2. 重庆市第三人民医院肿瘤科,重庆,400014
  3. 重庆医科大学基础医学院生化教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.04.013
  CLC： R734.2
- Published：2000，
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[1]杜铭,李静,曾昭淳,李良彬,张雪梅.非小细胞肺癌组织p16基因缺失及突变的临床意义[J].重庆医科大学学报,2000(04):368-370.

Study on the deletion and mutation of p16 gene in human non-small cell lung cancer and its clinical significance. [J]. Journal of Chongqing Medical University (4):368-370(2000)
[1]杜铭,李静,曾昭淳,李良彬,张雪梅.非小细胞肺癌组织p16基因缺失及突变的临床意义[J].重庆医科大学学报,2000(04):368-370. DOI： 10.13406/j.cnki.cyxb.2000.04.013.

Study on the deletion and mutation of p16 gene in human non-small cell lung cancer and its clinical significance. [J]. Journal of Chongqing Medical University (4):368-370(2000) DOI： 10.13406/j.cnki.cyxb.2000.04.013.

摘要

目的　探讨人类非小细胞肺癌组织中p16基因缺失及突变情况及其与临床病理的关系。方法　应用控制模板DNA量的银染PCR-SSCP技术检测40例非小细胞肺癌手术切除标本中p16基因第2外显子。结果　40例中有13例发生纯合缺失，3例发生突变，缺失及突变率为40%。其缺失及突变率与肺癌的临床病理分期有密切关系（Ｐ<0.05）。结论　 p16基因的缺失及突变可能在非小细胞肺癌的发生、发展及转移中起重要作用。

Abstract

：Objective To study the relationship between the deletion and mutation of p16 gene and clinicopatho -logic manifestations of human non-small cell lung cancer(NSCLC). Methods Silver staining PCR-SSCP method of control amount of template DNA was used to detect exon 2 of p16 gene in human NSCLC tissue specimens. Results The deletion of p16 gene was identified in 13 out of 40 specimens. Of the 40 specimens

3 showed a variant band indicative of the mutation. There was significant correlation between the frequency of the deletion and mutation(40%) and clinicopathologic stage(Ｐ<0.05). Conclusion The deletion and mutation of p16 gene may play an important role in carcinogenesis

development and metastasis of human NSCLC.

关键词

非小细胞肺癌p16基因PCR-SSCP缺失突变

Keywords

non-small cell lung cancerp16 genePCR-SSCPDeletionmutation

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