Cloning of differentiation-related cDNA induced by matrine from K562 cell line

doi:10.13406/j.cnki.cyxb.2000.04.001

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Cloning of differentiation-related cDNA induced by matrine from K562 cell line

更新时间：2024-01-17

- Cloning of differentiation-related cDNA induced by matrine from K562 cell line
- Journal of Chongqing Medical University Issue 4, Pages: 337-339(2000)
- 作者机构：
  
  重庆医科大学临床生化教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.04.001
  CLC： R346
- Published：2000，
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[1]张彦,蒋纪恺,刘小珊,刘北忠,许相儒,何渝军.苦参碱诱导K562细胞分化相关cDNA的克隆[J].重庆医科大学学报,2000(04):337-339.

Cloning of differentiation-related cDNA induced by matrine from K562 cell line. [J]. Journal of Chongqing Medical University (4):337-339(2000)
[1]张彦,蒋纪恺,刘小珊,刘北忠,许相儒,何渝军.苦参碱诱导K562细胞分化相关cDNA的克隆[J].重庆医科大学学报,2000(04):337-339. DOI： 10.13406/j.cnki.cyxb.2000.04.001.

Cloning of differentiation-related cDNA induced by matrine from K562 cell line. [J]. Journal of Chongqing Medical University (4):337-339(2000) DOI： 10.13406/j.cnki.cyxb.2000.04.001.

摘要

目的　克隆苦参碱诱导人白血病K562细胞分化的相关基因，以求了解白血病细胞分化及苦参碱的作用机制。方法　以人白血病细胞K562为研究对象，在苦参碱诱导其分化的基础上，利用mRNA差异显示技术对K562细胞在苦参碱诱导前及诱导后48小时的基因表达情况进行分析。结果　在苦参碱诱导前后的K562细胞之间存在明显的基因表达差异，一些基因在诱导后表达增强，而一些基因经苦参碱作用后表达减弱甚至完全抑制。经克隆和筛选获得部分表达差异的cDNA片段，为进一步研究苦参碱的诱导分化机制及肿瘤细胞发生和分化机制打下了基础。结论　苦参碱具有调控分化相关基因表达的作用，苦参碱诱导K562细胞分化是多基因共同参与的过程。

Abstract

：Objective To clone differentiation-related genes of human leukemia K562 cells

and to study the mechanism of differentiation of K562 cells induced by matrine. Methods Differentially expressed genes between K562 cells and their matrine-treatment cells have been investigated by mRNA differential display technique. Results There were obvious differences in gene expression of K562 cells before and after matrine treatment. The expression of some genes was decreased or inhibited while that of other genes was active. Conclusion Matrine plays an important role in regulating the differentiation-related gene expression. The differentiation induced by matrine is a complex process with multiple genes involved.

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差异显示PCR苦参碱K562细胞cDNA

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