Oxidation of high-density lipoproteins in vitro and protective effects of probucol

doi:10.13406/j.cnki.cyxb.2000.03.011

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Oxidation of high-density lipoproteins in vitro and protective effects of probucol

更新时间：2024-01-17

- Oxidation of high-density lipoproteins in vitro and protective effects of probucol
- Journal of Chongqing Medical University Issue 3, Pages: 253-255(2000)
- 作者机构：
  
  重庆医科大学临床学院心血管内科,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.03.011
  CLC： R363
- Published：2000，
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[1]胡厚源,陈运贞.HDL的体外氧化及丙丁酚的抗氧化作用[J].重庆医科大学学报,2000(03):253-255.

Oxidation of high-density lipoproteins in vitro and protective effects of probucol. [J]. Journal of Chongqing Medical University (3):253-255(2000)
[1]胡厚源,陈运贞.HDL的体外氧化及丙丁酚的抗氧化作用[J].重庆医科大学学报,2000(03):253-255. DOI： 10.13406/j.cnki.cyxb.2000.03.011.

Oxidation of high-density lipoproteins in vitro and protective effects of probucol. [J]. Journal of Chongqing Medical University (3):253-255(2000) DOI： 10.13406/j.cnki.cyxb.2000.03.011.

摘要

目的探讨高密度脂蛋白（ＨＤＬ）的体外氧化过程及丙丁酚对ＨＤＬ氧化的抑制作用。方法采用一次性密度梯度超速离心法获取人血清ＨＤＬ，以ＣＵ～２＋介导其氧化，荧光法检测样品中硫代巴比妥酸反应产物（ＴＢＡＲＳ）的含量，用于判定氧化效果。结果Ｃｕ２＋可有效地介导ＨＤＬ的体外氧化，并在一定范围内呈浓度和时间依赖性；预先加入丙丁酚可有效阻止ＨＤＬ的体外氧化，５μｍｏｌ／Ｌ丙丁酚即有显著的抗氧化作用。结论ＨＤＬ亦易于发生氧化修饰，抗氧化剂丙丁酚可明显抑制由Ｃｕ２＋介导的ＨＤＬ体外氧化。

Abstract

Objective To investigate the oxidation of high-density lipoproteins(HDL) in vitro and protective effects of probucol. Methods Human plasma lipoproteins were separated by one-step ultracentrifugation with a simple density-gradient. The in vitro oxidation of HDL was mediate by CuSO4

the oxidative effect of Cu2+ and the antioxidation effect of probucol were assayed simultaneously by thiobarbituric acid reactive substances (TBARS). Results (1) Cu2+ could effectively mediated the oxidation of HDL in vitro

which was both dose-dependent and time-dependent with Cu2+ within definite ranges.(2) The oxidation of HDL in vitro could be inhibited by probucol

and 5μmol/L probucol was effective obviously. Conclusion HDL is susceptible to oxidation and probucol can inhibit the Cu2+ mediated oxidation of HDL in vitro.

关键词

脂蛋白丙丁酚

Keywords

LipoproteinsProbucol

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YE Xiulan

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Related Institution

Department of Endocrinology,the First Affiliated Hospital,Chongqing Medical University

重庆医科大学附属第一医院内分泌科

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