Effects of recombinant BCG-Sj26GST vaccine of schistome on the expression of TNF-α mRNA during the formation of egg granulomas in the liver of mice

doi:10.13406/j.cnki.cyxb.2000.03.001

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Effects of recombinant BCG-Sj26GST vaccine of schistome on the expression of TNF-α mRNA during the formation of egg granulomas in the liver of mice

更新时间：2024-01-17

- Effects of recombinant BCG-Sj26GST vaccine of schistome on the expression of TNF-α mRNA during the formation of egg granulomas in the liver of mice
- Journal of Chongqing Medical University Issue 3, Pages: 225-227(2000)
- 作者机构：
  
  1. 重庆医科大学临床学院传染病寄生虫病研究所
  2. 同济医科大学寄生虫学教研室,400016
  3. 武汉市卫生防疫站消毒科
  4. ,430022
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.03.001
  CLC： R392
- Published：2000，
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[1]李文桂,石佑恩,蒋霞,甘燕.血吸虫重组BCG-Sj26GST疫苗对小鼠肝脏虫卵肉芽肿形成过程中TNF-αmRNA表达的影响[J].重庆医科大学学报,2000(03):225-227.

Effects of recombinant BCG-Sj26GST vaccine of schistome on the expression of TNF-α mRNA during the formation of egg granulomas in the liver of mice. [J]. Journal of Chongqing Medical University (3):225-227(2000)
[1]李文桂,石佑恩,蒋霞,甘燕.血吸虫重组BCG-Sj26GST疫苗对小鼠肝脏虫卵肉芽肿形成过程中TNF-αmRNA表达的影响[J].重庆医科大学学报,2000(03):225-227. DOI： 10.13406/j.cnki.cyxb.2000.03.001.

Effects of recombinant BCG-Sj26GST vaccine of schistome on the expression of TNF-α mRNA during the formation of egg granulomas in the liver of mice. [J]. Journal of Chongqing Medical University (3):225-227(2000) DOI： 10.13406/j.cnki.cyxb.2000.03.001.

摘要

目的检测血吸虫重组ＢＣＧ－Ｓｊ２６ＧＳＴ疫苗对小鼠肝虫卵肉芽肿细胞内ＴＮＦ－ｍＲＮＡ表达的影响。方法用１０～６ＣＦＵ疫苗皮下注射和腹腔注射分别免疫ＢＡＬＢ／Ｃ鼠，免疫后８Ｗ用日本血吸虫尾蚴进行攻击感染，感染后６Ｗ剖杀小鼠，分离肝脏，运用原位杂交技术检测ＴＮＦ－α ｍＲＮＡ表达。结果疫苗免疫，尾蚴攻击后小鼠肝虫卵肉芽肿细胞内ＴＮＦ－α ｍＲＮＡ表达显著加强。结论血吸虫重组ＢＣＧ－Ｓｊ２６ＧＳＴ疫苗能促进宿主Ｔｈ１反应，加强宿主抗血吸虫感染的保护性免疫力。

Abstract

Objective To detect the effects of recombinant BCG-Sj26GST vaccine of schistosome on the expression of TNF-α mRNA in cells of egg granulomas in the liver of mice. Methods BALB/C mice were immunized subcutaneously and intraperitoneally by 10~6 CFU vaccine respectively

challenged with Sj cercariae on 8w of immunization and killed on 6w of infection to separate the liver to detect the expression of TNF-α mRNA by the technique of in situ hybridizaion. Results The expression of TNF-α mRNA in egg granulomas in the liver of mice increased obviously by the immunization with the vaccine against challenge with Sj cercariae. Conclusion Recombinant BCG-Sj26GST vaccine of the schistosome may promote host’s Th1 response and enhance its protective immunity against schistosomiasis

关键词

血吸虫疫苗TNF-α肝脏原位杂交

Keywords

Vaccine of schistomeTNF-αliverIn situ hybridization

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