The relationship between the common mode of HBV   immunological  marker in serum and the quantity of HBV- DNA

doi:10.13406/j.cnki.cyxb.2000.02.022

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The relationship between the common mode of HBV immunological marker in serum and the quantity of HBV- DNA

更新时间：2024-01-17

- The relationship between the common mode of HBV immunological marker in serum and the quantity of HBV- DNA
- Journal of Chongqing Medical University Issue 2, Pages: 169-170(2000)
- 作者机构：
  
  1. 重庆医科大学临床学院检验科,400016
  2. 重庆医科大学临床学院药剂科,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.02.022
  CLC： R446.6
- Published：2000，
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[1]严立,何芳,张玉洪.乙肝病毒标志物常见模式与HBV-DNA检出量的比较分析[J].重庆医科大学学报,2000(02):169-170+173.

The relationship between the common mode of HBV immunological marker in serum and the quantity of HBV- DNA. [J]. Journal of Chongqing Medical University (2):169-170(2000)
[1]严立,何芳,张玉洪.乙肝病毒标志物常见模式与HBV-DNA检出量的比较分析[J].重庆医科大学学报,2000(02):169-170+173. DOI： 10.13406/j.cnki.cyxb.2000.02.022.

The relationship between the common mode of HBV immunological marker in serum and the quantity of HBV- DNA. [J]. Journal of Chongqing Medical University (2):169-170(2000) DOI： 10.13406/j.cnki.cyxb.2000.02.022.

摘要

目的 :探讨乙肝病毒免疫标志物常见模式与HBV -DNA量的关系。方法 :采用荧光定量PCR法对 15 0份测定的血清标本进行乙肝病毒标志物与HBV -DNA定量测定。结果 :2 1份HBsAg、HBeAg、HBcAb阳性标本

HBV -DNA全部阳性

平均拷贝数为 3.5× 10 8;6份HBsAg、HBeAg阳性的标本也均为阳性

平均拷贝数为 1.2× 10 9;2 7例HBsAg、HBeAb、HB cAb阳性的标本中HBV -DNA阳性 12例

平均拷贝数 7.5× 10 6;2 4例HBsAg、HBcAb阳性的模式中阳性 8例

平均拷贝数 8.5× 10 5;其它HBsAb阳性和全阴性血清HBV -DNA都呈阴性。结论 :提示FQ -PCR可以检测HBV的真实感染和复制状态

对于乙型肝炎的临床诊断

治疗方案的选择和疗效观察有较大的指导意义

Abstract

Objective:To study the relationship between the common mode of HBV immunological marker in serum and the quantity of HBV-DNA. Methods:150 samples were detected by fluorescence quantitative PCR (FQ-PCR) and ELISA kit respectively. Results:In 21HBsAg+/HBeAg+/HbcAb+samples

the FQ-PCR were all positive with 3.5×10 8copies on average

in 6 HBsAg+/HBeAg+samples

the HBV-DNA were all positive and the mean was 1.2×10 9copies. in 27 HBsAg+/HBeAb+/HBcAb+samples 12 samples were positive tested FQ-PCRwith 7.5×10 6 copies

on average; In 24 HBsAg+/HBcAb+samples

8 samples were positive and the amount was 8.5×10 5

the residual HBsAb+and HBsAg -/HBsAb-/HBeAg-/HBeAb-/HBcAb- samples were negative by FQ-PCR. Conclusion:This indicates the FQ-PCR can be used to detect the true state of HBV infection and its replication. The FQ-PCR is helpful to diagnose the hepatitis B

and to select the therapeutic project and to monitor the medication.

关键词

乙型肝炎病毒标志物模式定量聚合酶链反应荧光光度测量法

Keywords

hepatitis B virusmarkerModeQuantitative PCRFluorphotimetry

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