Immobilized trypsin with compound silica

doi:10.13406/j.cnki.cyxb.2000.02.016

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Immobilized trypsin with compound silica

更新时间：2024-01-17

- Immobilized trypsin with compound silica
- Journal of Chongqing Medical University Issue 2, Pages: 154-155(2000)
- 作者机构：
  
  1. 重庆医科大学临床学院检验科,400016
  2. 华西医科大学药学生化教研室,610044
  3. 重庆医科大学临床学院检验科!400016华西医科大学药学生化教研室,610044
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.02.016
  CLC： TQ920.1
- Published：2000，
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[1]陈辉,杨继虞.以复合硅胶为载体的胰蛋白酶的固定化[J].重庆医科大学学报,2000(02):154-155+211.

Immobilized trypsin with compound silica. [J]. Journal of Chongqing Medical University (2):154-155(2000)
[1]陈辉,杨继虞.以复合硅胶为载体的胰蛋白酶的固定化[J].重庆医科大学学报,2000(02):154-155+211. DOI： 10.13406/j.cnki.cyxb.2000.02.016.

Immobilized trypsin with compound silica. [J]. Journal of Chongqing Medical University (2):154-155(2000) DOI： 10.13406/j.cnki.cyxb.2000.02.016.

摘要

目的 :制备固定化胰蛋白酶

用于转肽反应。方法 :以涂敷了壳聚糖的硅胶为复合载体

以系统全面反馈优化技术进行优化制备固定化胰蛋白酶。结果 :所制备的固定化胰蛋白酶平均酶活性为 2 2 82U/ g

酶活性回收在 9%以上。结论 :制备的固定化酶的单位酶活性高

带入转肽反应体系的水分少

能有效地应用于转肽反应之中

Abstract

Objective:An immobilized trypsin (ITP) is prepared for transpeptidation. Methods:The silica coated chitosan is used as compound carrier

and the process is optimized by a systemic and all-around feedback optimization technique. Results:The average activity of ITP is as high as 2282U/g

and the recovery rate of activity is 9%. Conclusion:The activity per gramme ITP is very high

and it carries a little water to system of transpeptidation

so it was used for transpeptidation successfully.

关键词

硅胶壳聚糖固定化胰蛋白酶优化技术

Keywords

SilicachitosanImmobilized trypsinOptimization technique

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Key Laboratory of Medical Diagnostics of Ministry of Education,Department of Medicine

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