Inhibitive effect of ribozyme gene and effect of cisplatin  on the expression of Bcl--2 in SK-N-SH

doi:10.13406/j.cnki.cyxb.2000.02.005

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Inhibitive effect of ribozyme gene and effect of cisplatin on the expression of Bcl--2 in SK-N-SH

更新时间：2024-01-17

- Inhibitive effect of ribozyme gene and effect of cisplatin on the expression of Bcl--2 in SK-N-SH
- Journal of Chongqing Medical University Issue 2, Pages: 123-124(2000)
- 作者机构：
  
  1. 重庆医科大学第二临床学院神经外科,400010
  2. 重庆医科大学临床学院生化教研室,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.02.005
  CLC： R739.41
- Published：2000，
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[1]程远,王世波,冯涛.核酶抑制神经母细胞瘤Bcl-2的表达及增强顺铂敏感性的实验研究[J].重庆医科大学学报,2000(02):123-124+127.

Inhibitive effect of ribozyme gene and effect of cisplatin on the expression of Bcl--2 in SK-N-SH. [J]. Journal of Chongqing Medical University (2):123-124(2000)
[1]程远,王世波,冯涛.核酶抑制神经母细胞瘤Bcl-2的表达及增强顺铂敏感性的实验研究[J].重庆医科大学学报,2000(02):123-124+127. DOI： 10.13406/j.cnki.cyxb.2000.02.005.

Inhibitive effect of ribozyme gene and effect of cisplatin on the expression of Bcl--2 in SK-N-SH. [J]. Journal of Chongqing Medical University (2):123-124(2000) DOI： 10.13406/j.cnki.cyxb.2000.02.005.

摘要

目的 :研究抗Bcl- 2mRNA的锤头型核酶（RibozymeRZ）基因抑制神经母细胞瘤（SK -N -SH）生长以及增强化疗药物顺铂的敏感性。方法 :（1）应用电穿孔法

分别将整合有抗Bcl- 2mRNA核酶的缺陷性逆转录病毒载体 pDOR -RZ和空载体pDOR -neo导入SK -N -SH细胞。（2 ）用免疫细胞染色法定性观察肿瘤细胞内Bcl- 2蛋白的表达 ;用间接免疫荧光染色法和流式细胞仪（FCM）测定Bcl- 2蛋白含量。（3）MTT法检测化疗药物顺铂敏感性。结果 :（1）转染RZ组Bcl- 2蛋白合成受到抑制

（2 ）顺铂增敏试验表明顺铂对转RZ组杀伤作用增强。结论 :将抗Bcl- 2核酶基因转入神经母细胞内

（1）可明显抑制肿瘤细胞内Bcl- 2蛋白的合成

促进瘤细胞死亡 ;（2 ）增强其对化疗药物顺铂的敏感性

Abstract

Objective: To observe whether tumor cell can be inhibited and the sensitivity of SK-N-SH cell transferred to cisplatin can be increased through transducting a ribozyme against Bcl- 2 into neuroblastoma cell line (SH-N-SH).Methods: pDOR-RZ retroviral vector was transduced into neuroblastoma cell by electroporation. By immunocytochemistry dyeing methods

the changes of Bcl- 2 expression were observed ; and the sensitivity of cisplatin to transferred cell was quantified by a modified 2

5-diphenyl tetrazolium bromide (MTT) colorimetric assay.Results: (1) the synthesis of BCL-2 protein was inhibited; (2) the sensitivity of the cell with RZ to cisplatin was increased. Conclusion:The ribozyme gene against Bcl-2 can inhibit the synthesis of Bcl-2 protein and tumor growth

and increase the sensitivity of chemotherapeutic agent.

关键词

核酶Bcl-2化疗基因治疗

Keywords

RibozymeBcl-2GenetherapyChemotherapeutant

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