Effect of autocrine IL-4 on killing activity of CTL induced by K<sub>562</sub>

ZHAO Xiao-dong; et al

doi:10.13406/j.cnki.cyxb.2000.01.001

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Effect of autocrine IL-4 on killing activity of CTL induced by K₅₆₂

更新时间：2024-01-17

- Effect of autocrine IL-4 on killing activity of CTL induced by K₅₆₂
- Journal of Chongqing Medical University Issue 1, Pages: 1-3(2000)
- 作者机构：
  
  重庆医科大学儿科学院免疫室,400014
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.01.001
  CLC： R730.3
- Published：2000，
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[1]赵晓东,李成荣,杨锡强,蒋利萍,王莉佳.自分泌IL-4对K_(562)细胞诱导的CTL杀伤活性的影响[J].重庆医科大学学报,2000(01):1-3.

ZHAO Xiao-dong, et al. Effect of autocrine IL-4 on killing activity of CTL induced by K₅₆₂. [J]. Journal of Chongqing Medical University (1):1-3(2000)
[1]赵晓东,李成荣,杨锡强,蒋利萍,王莉佳.自分泌IL-4对K_(562)细胞诱导的CTL杀伤活性的影响[J].重庆医科大学学报,2000(01):1-3. DOI： 10.13406/j.cnki.cyxb.2000.01.001.

ZHAO Xiao-dong, et al. Effect of autocrine IL-4 on killing activity of CTL induced by K₅₆₂. [J]. Journal of Chongqing Medical University (1):1-3(2000) DOI： 10.13406/j.cnki.cyxb.2000.01.001.

摘要

探讨ＩＬ－４基因修饰对Ｋ５６２细胞诱导的肿瘤特异性ＣＴＬ杀伤活性的促进作用及可能机理。采用逆转录病毒体ＬＸＳＮ将ＩＬ－４ｃＤＮＡ导入Ｋ５６２细胞，经Ｇ４１８筛选后获得表达ＩＬ－４蛋白的Ｋ５６２克隆。以野生型和载体修饰的相应细胞为对照，检测了ＩＬ－４基因修饰Ｋ５６２细胞诱导的Ｔ细胞增殖反应，用５１铬（５１Ｃｒ）释放法检测ＣＴＬ杀伤活性。结果发现ＩＬ－４基因修饰的Ｋ５６２细胞诱导的Ｔ细胞增殖反应及ＣＴＬ杀伤活性均明显高于对照组。提示ＩＬ－４基因修饰可能通过上调Ｔ细胞功能促进肿瘤特异性ＣＴＬ杀伤活性。

Abstract

To observe the effect and possible mechanism of IL-4 gene modification on killing activity of K562 -specific CTL

IL-4 cDNA was transduced into K562 cell via the retroviral vector-mediated gene transfer method. G418-resistant clones were obtained and expression of IL-4 of IL-4 gene-engineered and parental K562 cells was determined. Meanwhile

proliferation of normal PBMC cells were assayed by 3H-TdR incorporation and the 51Cr release test

respectively. The results showed that proliferation of normal PBMC and killing activity of CTL induced by IL-4 positive K562 cells were greatly increased. It was concluded that IL-4 gene modification is capable of upregulating the killing activity of K562-specific CTL by possibly enhancing the T lylnphocyte function.

关键词

IL-4基因治疗白血病

Keywords

Interleukin-4gene therapyleukemia

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Effect of autocrine IL-4 on killing activity of CTL induced by K562

DOI：10.13406/j.cnki.cyxb.2000.01.001

摘要

Abstract

关键词

Keywords

references

Effect of autocrine IL-4 on killing activity of CTL induced by K₅₆₂