Inhibitory effects of tetrandrine on production of phospholipase A<sub>2</sub>, prostaglandin synthase 2 and prostaglandin E, in U937 cells induced by lipopolysaccharide

Zhao Shuming; et al

doi:10.13406/j.cnki.cyxb.1999.04.011

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Inhibitory effects of tetrandrine on production of phospholipase A₂, prostaglandin synthase 2 and prostaglandin E, in U937 cells induced by lipopolysaccharide

更新时间：2024-01-17

- Inhibitory effects of tetrandrine on production of phospholipase A₂, prostaglandin synthase 2 and prostaglandin E, in U937 cells induced by lipopolysaccharide
- Journal of Chongqing Medical University Issue 4, Pages: 368-370(1999)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1999.04.011
  CLC： R392-33
- Published：1999，
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[1]赵树铭,彭永正,蒋纪恺,康格非,向国春.磷脂酶A_2抑制剂的研究──粉防己碱对U937细胞磷脂酶A_2和前列腺素生成的影响[J].重庆医科大学学报,1999(04):368-370.

Zhao Shuming, et al. Inhibitory effects of tetrandrine on production of phospholipase A₂, prostaglandin synthase 2 and prostaglandin E, in U937 cells induced by lipopolysaccharide. [J]. Journal of Chongqing Medical University (4):368-370(1999)
[1]赵树铭,彭永正,蒋纪恺,康格非,向国春.磷脂酶A_2抑制剂的研究──粉防己碱对U937细胞磷脂酶A_2和前列腺素生成的影响[J].重庆医科大学学报,1999(04):368-370. DOI： 10.13406/j.cnki.cyxb.1999.04.011.

Zhao Shuming, et al. Inhibitory effects of tetrandrine on production of phospholipase A₂, prostaglandin synthase 2 and prostaglandin E, in U937 cells induced by lipopolysaccharide. [J]. Journal of Chongqing Medical University (4):368-370(1999) DOI： 10.13406/j.cnki.cyxb.1999.04.011.

摘要

探讨粉防己碱对培养的Ｕ９３７细胞中花生四烯酸瀑布系统产生前列腺素的影响。以Ｕ９３７细胞株为模型，加入内毒素（ＬＰＳ）处理，以及粉防己碱预处理后再加入ＬＰＳ处理，观察Ⅱ－型磷脂酶Ａ２（Ⅱ—ＰＬＡ２）、胞液型磷脂酶Ａ２－（ｃＰＬＡ２）和环氧合酶－２（ＣＯＸ—２）以及前列腺素Ｅ２（ＰＧＥ２）的变化。结果发现ＬＰＳ可刺激Ｕ９３７细胞中Ⅱ－ＰＬＡ２、ｃＰＬＡ２和ＣＯＸ—２以及ＰＧＥ２的产生增加，粉防己碱则可不同程度地抑制它们的增高。粉防己碱具有降低内毒素引发的Ⅱ—ＰＬＡ２、ｃＰＬＡ２和ＣＯＸ—２以及ＰＧＥ２产生的作用。

Abstract

The purpose of thin study was to investigate the inhibitory effects of tetrandrine on the production ofII-phospholipase A

（II - PLA

）

cytosolic phospholipase A

（cPLA

）

cyclo - oxygenase 2

COX - 2 （orprostaglandin synthase 2） and prostaglandin E

（PGE

） in U937 cells induced by lipopolysacoharide（LPS）. Cultured U937 cells were separately treated by adding LPS and tetrandrine. II-PLA

activity and the levels of cPLA

COX-2 and PGE

in U937 cells were analyzed by radioactive assay or ELISA. It was found that LPS couldincrease the activity of II-PLA

and stimulate U937 cells to produce more cPLA

COX-2 and PGE

.Tetrandrine could inhibit the increase of these indicators induced by LPS to different degrees. Our findingssuggest that tetrandrine plays the role of anti- inflammation through inhibiting the production of phospholipaseA

prostaglandin synthase 2 and prostaglandin by U937 cells.

关键词

粉防己碱磷脂酶A2环氧合酶－2前列腺素E2

Keywords

TetrandrinePhospholipase A2Cyclooxygenase 2prostaglandin E2

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⁰

Inhibitory effects of tetrandrine on production of phospholipase A2, prostaglandin synthase 2 and prostaglandin E, in U937 cells induced by lipopolysaccharide

DOI：10.13406/j.cnki.cyxb.1999.04.011

摘要

Abstract

关键词

Keywords

references

Inhibitory effects of tetrandrine on production of phospholipase A₂, prostaglandin synthase 2 and prostaglandin E, in U937 cells induced by lipopolysaccharide