Studies on nitric oxide synthase gene expression in acute experimental pancreatitis in rats

doi:10.13406/j.cnki.cyxb.1999.04.005

您当前的位置：

首页 >

文章列表页 >

Studies on nitric oxide synthase gene expression in acute experimental pancreatitis in rats

更新时间：2024-01-17

- Studies on nitric oxide synthase gene expression in acute experimental pancreatitis in rats
- Journal of Chongqing Medical University Issue 4, Pages: 350-354(1999)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1999.04.005
  CLC： R576
- Published：1999，
扫描看全文
[1]冯莉,王丕龙,胥飚,刘新才.实验性急性胰腺炎性胰腺组织—氧化氮合酶基因表达的研究[J].重庆医科大学学报,1999(04):350-354.

Studies on nitric oxide synthase gene expression in acute experimental pancreatitis in rats. [J]. Journal of Chongqing Medical University (4):350-354(1999)
[1]冯莉,王丕龙,胥飚,刘新才.实验性急性胰腺炎性胰腺组织—氧化氮合酶基因表达的研究[J].重庆医科大学学报,1999(04):350-354. DOI： 10.13406/j.cnki.cyxb.1999.04.005.

Studies on nitric oxide synthase gene expression in acute experimental pancreatitis in rats. [J]. Journal of Chongqing Medical University (4):350-354(1999) DOI： 10.13406/j.cnki.cyxb.1999.04.005.

摘要

探讨急性胰腺炎（ＡＰ）发病机制与一氧化氮（ＮＯ）的关系，用十二指肠闭拌法诱导大鼠实验性ＡＰ模型，并对ＡＰ形成后检测不同时相的胰腺组织一氧化氮合酶（ＮＯＳ）ｍＲＮＡ，以了解ＡＰ病程中ＮＯＳ基因表达水平的动态变化。４８只大鼠随机分四组。一组为对照组，三组为实验组。实验组分别于术后２４、３６、４８ｈ处死检测血清淀粉酶，取胰腺组织进行逆转录聚合酶链反应（ＲＴ—ＰＣＲ），检测ＮＯＳＣＤＮＡ含量。结果：术后２４ｈ已形成出血坏死型ＡＰ，血清淀粉酶比对照组显著增高（Ｐ＜０．０１），而ＮＯＳｍＲＮＡ水平与对照组相比无明显改变。至３６ｈ时ＮＯＳｍＲＮＡ水平显著增高至对照组的１．２或１．５倍（Ｐ＜０．０１），其后又开始下降。结论：大鼠实验性ＡＰ胰腺组织ＮＯＳ基因表达在术后３６ｈ达高峰，４８ｈ时下降，ＮＯ在ＡＰ发病过程中有一定作用，其确切机理有待进一步探讨。

Abstract

The study was undertaken for the purpose of investigating tile relationship between acute pancreatitis (AP)and nitris oxied(NO). The animals of AP were killed at the period of 24

48 hours after induction of AP. Non-operated onrmal rats(n=12) were served as control.The degree of serum amyase levels and histopathologicalterations were investigated. At the same time the levels of nitric oxide synthase (NOS) mRNA in pancreatictissues were measured by the method of reverse transcription polymerase chain reaction(RT- PCR)

in order toreflect NOS gene expression levels. Primers were designed referring to brain NOS CDNA sequence of rats regionswhich were highly kindred to human endothelial derived NOS CDNA were seleced

actin as on innerreference. Restits:Hemorrhagic necrotizing AP was induced by the time of 24h after operation

which was similarto that found in man. As compared with control group

serum amylase level was markedly increased at24h(P<0.01)

whereas NOS mRNA level was markedly increased to 1.2 or 1.5 times the level of control group (P<0.01)

forming a peak of NOS gene expression level

then was decreased at 48h. Our preliminary results suggestthat NO may have a role in the pathogenesis of AP.

关键词

胰腺炎一氧化氮合酶基因表达聚合酶链反应

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

THE HIGH EXPRESSION OF HBV CORE ANTIGEN IN E.COLI

Retroviral vector - mediated expression of human interleukin-4 gene in human tumor cells

Construction of a mutant human proinsulin gene with signal peptide code and detection of its expression in COS-7 cells

Expression of leptin and leptin receptor system in the endometrium of mouse in the implantation process

Cloning of human telomerase reverse transcriptase gene promoter and analysis of specific activities in human hepatocellular carcinoma cells

Related Author

G.B.Thornton

张定凤

贾小平

黄爱龙

蒋丽萍

杨锡强

李成荣

赵晓东

Related Institution

重庆医科大学病毒性肝炎研究所

美国Johnson＆Johnson药理研究所副所长

儿科学院免疫室

Department of Endocrinology,College of Clinical Medicine,Chongqing Medical University

山西医科大学第一医院

⁰