Preparation and application of human IL - 3cDNA probe

Wang Yaping; et al

doi:10.13406/j.cnki.cyxb.1999.04.001

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Preparation and application of human IL - 3cDNA probe

更新时间：2024-01-17

- Preparation and application of human IL - 3cDNA probe
- Journal of Chongqing Medical University Issue 4, Pages: 337-340(1999)
- 作者机构：
  
  中国医学科学院血液学研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1999.04.001
  CLC： Q782
- Published：1999，
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[1]王亚平,王璐,郑敏,吴宏,姜蓉,王勇.人IL—3cDNA探针制备及应用[J].重庆医科大学学报,1999(04):337-340.

Wang Yaping, et al. Preparation and application of human IL - 3cDNA probe. [J]. Journal of Chongqing Medical University (4):337-340(1999)
[1]王亚平,王璐,郑敏,吴宏,姜蓉,王勇.人IL—3cDNA探针制备及应用[J].重庆医科大学学报,1999(04):337-340. DOI： 10.13406/j.cnki.cyxb.1999.04.001.

Wang Yaping, et al. Preparation and application of human IL - 3cDNA probe. [J]. Journal of Chongqing Medical University (4):337-340(1999) DOI： 10.13406/j.cnki.cyxb.1999.04.001.

摘要

采用多聚酶连反应（ＰＣＲ）扩增人ＩＬ—３ｃＤＮＡ片段，以地高辛（Ｄｉｇｏｘｉｇｅｎｉｎ—１１—ｄＵＴＰ）为标记物，随机引物法标记ＩＬ—３ｃＤＮＡ探针。探针经斑点杂交和核酸原位杂交证明：用制备ＩＬ—３ｃＤＮＡ探针检测细胞ＩＬ－－３ｍＲＮＡ表达具有特异性强，灵敏度高，使用简便，杂交结果直观，探针可较长时间保存和无放射性污染等优点。

Abstract

By using the techniques of polymerase chain reaction(PCR)

human IL - 3cDNA probe was prepared with pUC18- IL- 3 plasmid

labeled with digoxigenin - 11dUTP and identified with dot blot. We detected the expressionof IL- 3 mRNA in murine bone marrow stromal cells

human fetal thymocytes and splenic cells by nucleisitu hybridization. The results indicated that the prepared IL-3cDNA probe can provide the sensitivitysimplicity of handling needed to replace radioactiassays.

关键词

IL—3cDNA探针核酸原位杂交聚合酶连反应

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