INVESTIGATIONS ON ISOLATION-AND CULTURE IN VITRO OF NEWBORN PORCINE PANCREATIC ISLETS

doi:10.13406/j.cnki.cyxb.1993.04.003

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INVESTIGATIONS ON ISOLATION-AND CULTURE IN VITRO OF NEWBORN PORCINE PANCREATIC ISLETS

更新时间：2024-01-17

- INVESTIGATIONS ON ISOLATION-AND CULTURE IN VITRO OF NEWBORN PORCINE PANCREATIC ISLETS
- Journal of Chongqing Medical University Issue 4, Pages: 258-260(1993)
- 作者机构：
  
  重庆医科大学第一医院内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1993.04.003
  CLC：
- Published：1993，
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[1]李佐强,何军,舒昌达.新生猪胰岛分离及体外培养研究[J].重庆医科大学学报,1993(04):258-260+334.

INVESTIGATIONS ON ISOLATION-AND CULTURE IN VITRO OF NEWBORN PORCINE PANCREATIC ISLETS. [J]. Journal of Chongqing Medical University (4):258-260(1993)
[1]李佐强,何军,舒昌达.新生猪胰岛分离及体外培养研究[J].重庆医科大学学报,1993(04):258-260+334. DOI： 10.13406/j.cnki.cyxb.1993.04.003.

INVESTIGATIONS ON ISOLATION-AND CULTURE IN VITRO OF NEWBORN PORCINE PANCREATIC ISLETS. [J]. Journal of Chongqing Medical University (4):258-260(1993) DOI： 10.13406/j.cnki.cyxb.1993.04.003.

摘要

本文介绍一种以国产胶原酶消化与组织培养获得新生猪胰岛样细胞团（ICC）的方法。每个新生猪胰腺经浓度为6mg/ml胶原酶消化7—9min

再体外培养8天后

可获取2000～3000ICC。同时

新生猪ICC内β细胞不但快速或慢速相胰岛素分泌功能良好

而且其超微结构亦正常

说明新生猪ICC可作为研究胰岛细胞分化与发育以及异种移植治疗糖尿病的新来源

Abstract

A method for the in vitro production of newborn porcine pancreatic islellike cell clusters( ICC) by collagenase digestion and tissue culture was introduced in this paper. 2000-3000 ICC may be isolated from each newborn porcine panrreas which was digested by 6mg/ml collagenase concentration for 7-9 min and cultured for 8 days. Meanwhile

the functions both acute and chronic phase insulin secretion from the cells of the newborn porcine ICC were normal

also the fine structure of them ware excellent. It has been demonstrated that the newborn porcine ICC may be as a potential source both the studies in islet cell growth

and differentiation and xenograft transplantation ic treatment of diabetes

关键词

胰岛样细胞团胰岛素释放胰岛素含量

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