A STUDY OF METHODS FOR CULTIVATION AND ISOLATION OF GHLAMYDIA TRACHOMATlS IN GENITOURINARY TRACT

doi:10.13406/j.cnki.cyxb.1993.03.023

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A STUDY OF METHODS FOR CULTIVATION AND ISOLATION OF GHLAMYDIA TRACHOMATlS IN GENITOURINARY TRACT

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- A STUDY OF METHODS FOR CULTIVATION AND ISOLATION OF GHLAMYDIA TRACHOMATlS IN GENITOURINARY TRACT
- Journal of Chongqing Medical University Issue 3, Pages: 229-232(1993)
- 作者机构：
  
  重庆医科大学微生物学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1993.03.023
  CLC：
- Published：1993，
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[1]朱道银,朱汉林,李伟,王毅,秦亚恬,裴润芳.泌尿生殖道沙眼衣原体分离培养方法探讨[J].重庆医科大学学报,1993(03):229-232.

A STUDY OF METHODS FOR CULTIVATION AND ISOLATION OF GHLAMYDIA TRACHOMATlS IN GENITOURINARY TRACT. [J]. Journal of Chongqing Medical University (3):229-232(1993)
[1]朱道银,朱汉林,李伟,王毅,秦亚恬,裴润芳.泌尿生殖道沙眼衣原体分离培养方法探讨[J].重庆医科大学学报,1993(03):229-232. DOI： 10.13406/j.cnki.cyxb.1993.03.023.

A STUDY OF METHODS FOR CULTIVATION AND ISOLATION OF GHLAMYDIA TRACHOMATlS IN GENITOURINARY TRACT. [J]. Journal of Chongqing Medical University (3):229-232(1993) DOI： 10.13406/j.cnki.cyxb.1993.03.023.

摘要

本文采用放线菌酮处理的McCoy细胞分离培养泌尿生殖道沙眼衣原体。450例宫颈炎

盆腔炎患者培养阳性234人（52%）

30例男性尿道炎患者培养阳性15人（50％）

提示沙眼衣原体引起的泌尿生殖道感染在国内十分常见。249份阳性中二次传代阳性73人（29.3％）

放线菌酮最适浓度为1～4μg/ml

经离心处理的标本包涵体形成数明显高于不离心标本。用放线菌酮处理细胞、离心、二次传代在分离培养该病原体中是必不可少的步骤

Abstract

Chlamydia trachomatis in genitourinary tract were cultivated and isolated by culturing McCoy cells treated with cycloheximide. Of the 450 cases of cervicitis Or inflammatory diseases

234(52%) positive cultures were detected. 15(50%) positive cultures were detected in 30 cases of urethritis in men. It was suggested that genitourinary infacfions caused by C.trachomatis were common in our country. Of 249 positive cultures

73(29.3%) were detected by the second passage. Cycloheximide optimal concentration in inoculation media was 1-4ng/ml. Inclusion number of the samples centrifugad at 2000g was higher than that of uncentrifuged samples. Cells treated with cycloheximide

cefltrifugation

and a second passage were necessary for cultivation and isolation of the pathogen

关键词

沙眼衣原体沙眼衣原体培养泌尿生殖道感染

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