EVALUATION ON THE METROLOGY OF IMPROVED ASSAY METHOD FOR 6 MARKS ON HBV USING FINGER’S BLOOD AND MICRODETERMINATION ELlSA

doi:10.13406/j.cnki.cyxb.1993.03.022

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EVALUATION ON THE METROLOGY OF IMPROVED ASSAY METHOD FOR 6 MARKS ON HBV USING FINGER’S BLOOD AND MICRODETERMINATION ELlSA

更新时间：2024-01-17

- EVALUATION ON THE METROLOGY OF IMPROVED ASSAY METHOD FOR 6 MARKS ON HBV USING FINGER’S BLOOD AND MICRODETERMINATION ELlSA
- Journal of Chongqing Medical University Issue 3, Pages: 225-228(1993)
- 作者机构：
  
  1. 重庆医科大学检验系
  2. 重庆医科大学第一医院检验科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1993.03.022
  CLC：
- Published：1993，
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[1]刘国卿,罗云萍,曹唯希,康红,张玉洪,赖利华.手指全血微量ELISA法检测乙型肝炎病毒六项标志物的方法学评价[J].重庆医科大学学报,1993(03):225-228.

EVALUATION ON THE METROLOGY OF IMPROVED ASSAY METHOD FOR 6 MARKS ON HBV USING FINGER’S BLOOD AND MICRODETERMINATION ELlSA. [J]. Journal of Chongqing Medical University (3):225-228(1993)
[1]刘国卿,罗云萍,曹唯希,康红,张玉洪,赖利华.手指全血微量ELISA法检测乙型肝炎病毒六项标志物的方法学评价[J].重庆医科大学学报,1993(03):225-228. DOI： 10.13406/j.cnki.cyxb.1993.03.022.

EVALUATION ON THE METROLOGY OF IMPROVED ASSAY METHOD FOR 6 MARKS ON HBV USING FINGER’S BLOOD AND MICRODETERMINATION ELlSA. [J]. Journal of Chongqing Medical University (3):225-228(1993) DOI： 10.13406/j.cnki.cyxb.1993.03.022.

摘要

本文建立了手指全血微量ELISA法检测乙型肝炎病毒六项标记物的改良方法

并按照卫生部对乙肝标志物检测质控的要求对该方法作了系统的方法学评价

用该法和静脉血清常规法同时检测了240例乙肝六项标志物

两方法检测结果经配对资料显著性检验无显著性差异（P>0.05）。该方法突出的优点是用血量甚微

直接用全血加样

实验所用试剂、标本加样全部减半量反应

Abstract

In this paper

we reported the improved assay method for 6 marks on HBV using finger’s blood and microdetermination ELISA. It was evaluated with me-thology according to the quality control standard published by the Health Ministry. Two methods were compared using determination of serum of venous blood and using mirrodetermination of finger’s blood for dsctetion of 6 marks on HBV in 240 patients. Their identical ratioes were 93.8-98.3%and this method is better than others. It needs only 80ul finger’s blood and it doesn’t need serum separated from venous blood. During the experiment

only a half dose reagent and sample were used

关键词

乙肝标志物酶联免疫吸附试验

Keywords

Marks of HBVELISA

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Related Institution

Department of Clinical Laboratory, College of Clinical Medicine, Chongqing Medical University

重庆医科大学临床学院肾内科

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